摘要
目的探讨胰高血糖素样肽-1（glucagon—like peptide-1，GLP-1）对高糖环境诱导兔胸主动脉内皮细胞增殖凋亡的作用和HSP70表达的影响。方法胶原酶消化法培养兔胸主动脉内皮细胞，加入不同处理因素进行分组，TUNEL法测定内皮细胞凋亡，BRDU法测定内皮细胞增殖．Western blot法测定内皮细胞的HSP70表达水平。结果高糖（33mmol／L）培养24h后兔胸主动脉内皮细胞增殖（0．54±0．06）低于对照组（0．78±0．04），细胞凋亡率（36．43±6．85）％与HSP70的表达水平（0．94±0．11）高于对照组[（5．25±0．73）％与（0．29±0．03）]，差异有统计意义（P〈0．01）。GLP．1组与高糖组相比，内皮细胞增殖（0．62±0．06）增加。细胞凋亡率（10．13±1．19）％与HSP70表达水平（0．76±0．05）降低，差异有统计学意义（P〈0．05）。在exendin9-39组，内皮细胞的增殖、凋亡与HSP70表达与高糖组相比则无明显差异（P〉0．05）。结论GLP-1通过GLP-1受体依赖途径促进高糖条件下兔胸主动脉内皮细胞增殖，抑制细胞凋亡，减低高糖诱导HSP70表达水平。
Objective To observe the effect of GLP-1 on HSP70 expression of rabbit thoracic aortic endothelial cells at high glucose level. Methods Rabbit thoracic aortic endothelial ceils were isolated and cultured. Tunnel was used to assay endothelial cells apoptosis. Cell proliferation was analyzed with BUDR. The protein level of HSP70 were detected by Western blot. Results The proliferation of rabbit thoracic aortic endothelial ceils cultured under high glucose （33 mmol/L） decreased, （0. 54 ± 0. 06 vs 0.78 ± 0.04, P 〈 0. 01 ）. The apoptosis and the protein level of HSP70 increased, （36.43% ±6. 85% vs 5.25% ± 0. 73% ） and （0. 94 ± 0. 11 vs 0. 29 ± 0.03, all P 〈 0.01 ）. The proliferation of rabbit thoracic aortic endothelial cells （0. 62±0. 06） in the GLP-1 group increased, the apoptosis ratio （ 10. 13% ± 1.19% ） and HSP70 expression （ 0. 76± 0. 05 ） decreased significantly （ P 〈 0.05 ） compared with high glucose group. Conclusion GLP-1 can ameliorate high-glucose-induced rabbit thoracic aortic endothelial cells apoptosis and promote cell proliferation, attenuating high-glucose-induced level of HSPT0 expression through the GLP- 1 R-dependent pathways.
出处
《中华普通外科杂志》
CSCD
北大核心
2016年第8期632-635,共4页
Chinese Journal of General Surgery
基金
国家临床重点专科建设基金资助项目（国卫办医函[2013]544号）
福建省教育厅科技基金资助项目（JA11117）
