紫甘薯叶超声波提取工艺优化及其对小鼠肝脏保护作用研究

Extraction of Purple Sweet Potato Leaves and its Protective Effects on CCl_4-induced Acute Liver Injury of Mice

优化紫甘薯叶的超声波提取工艺,考察提取物对CCl_4诱导急性肝损伤小鼠的保护作用。采用3因素(乙醇浓度、超声功率、超声时间)3水平正交试验,以紫甘薯叶提取物清除羟基自由基能力为考察指标,优化紫甘薯叶超声波提取工艺参数,并用CCl_4建立急性肝损伤模型,考察不同剂量的紫甘薯叶超声波提取物(0.1 g/kg、0.3 g/kg)对肝损伤的影响,探讨紫甘薯叶超声波提取物的肝脏保护作用。结果表明,紫甘薯叶超声波提取最优提取工艺为:乙醇浓度70%,超声功率400 W,超声时间17 min。与模型组相比,紫甘薯叶超声波提取物能降低急性肝损伤小鼠血清中ALT活性及肝组织MDA含量,提高肝组织SOD活性和GSH水平,明显减轻肝损伤小鼠肝组织的损伤程度,表明紫甘薯叶超声波提取物对CCl_4诱导急性肝损伤小鼠具有保护作用。

The ultrasonic extraction process of purple sweet potato leaves was optimized and the protective effect of the extract on CCl4-induced acute hepatic injury of mice was investigated. The extraction process was optimized by orthogo- nal test with three factors （ ethanol concentration, ultrasonic power, and ultrasonic time） and three levels. The hydroxyl radical scavenging rate was used as investigation index. The protective effect of different doses （0.1 g/kg,0.3 g/kg） of purple sweet potato leaves extract on hepatic injury was investigated by the establishment of acute hepatic injury model using CCl4. The results showed that the optimal extraction conditions were as follows：ethanol concentration of 70%, ul- trasonic power of 400 W, ultrasonic time of 17 min. Compared with the model group, purple sweet potato leaves extract decreased the activities of serum ALT and hepatic MDA concentration, increased the activities of hepatic SOD and the level of hepatic GSH, and obviously relieved damage degree of liver tissue in the hepatic injury mice. These results indi- cated that purple sweet potato leaves extract had protective effect on CCl4-induced acute hepatic injuries of mice.