响应面法优化微波提取芦荟皮多糖及其抗氧化活性

Optimization of Microwave-Assisted Extraction of Polysaccharides from Aloe Peels by Response Surface Analysis and Its Antioxidant Activity

研究芦荟皮多糖的微波辅助提取工艺及其抗氧化能力。在单因素试验基础上,通过响应面分析法优化微波辅助提取工艺,建立可靠的数学模型。通过DPPH法、亚油酸体系法、羟自由基清除法研究其抗氧化活性。结果显示,芦荟皮多糖的最佳微波辅助提取条件为液料比31∶1(m L/g)、微波时间95 s、微波功率400 W、提取温度74℃,在此条件下芦荟皮多糖提取率为4.926%。所提芦荟皮多糖能够降低脂质过氧化物含量,清除DPPH自由基和羟自由基,具有较强的抗氧化能力,且在一定浓度范围,其抗氧化能力与粗提物浓度呈现一定的剂量效应关系。

The study focused on microwave-assisted extraction of polysaccharides from aloe peels by response surface methodology, and the antioxidant activity of polysaccharides from aloe peels was also evaluated. The extraction conditions of microwave-assisted of polysaccharides from Aloe peels were optimized by response surface methodology, its mathematical model was established, moreover, its antioxidant activity was also studied in vitro. Results showed that the optimal parameiers of microwave extraction were liquid-solid ratio of 31 ∶ 1（m L/g）, microwave time 95 s, microwave power 400 W, extraction temperature 74 ℃. Under the optimal conditions, the polysaccharide extraction rate was 4.926%. The antioxidant activity of polysaccharides from aloe peels was found in does dependent manner.