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紫色色杆菌来源的抗肿瘤抗生素SIPI-228的分离纯化及结构鉴定 被引量：1

Isolation,Purification and Structure Elucidation of an Anti-tumor Antibiotic SIPI-228 from Chromobacterium violaceum
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摘要 本室筛选得到一种由紫色色杆菌(Chromobacterium violaceum)所产的抗生素,将其命名为SIPI-228。本研究通过大孔树脂吸附、丙酮溶液梯度洗脱、乙酸乙酯萃取和无水乙醚析出等步骤,获得色谱纯度大于97%的SIPI-228精制品;并通过质谱、NMR分析,确定SIPI-228的结构与罗米地辛一致。 An anti-tumor antibiotic, named as SIPI-228, produced by Chromobacterium violaceum was screened and obtained in our lab. The compound SIPI-228 was purified by X-5 macroporous resin adsorption, gradient elution, extraction by ethyl acetate and precipitation by anhydrous ether, etc., with the purity over 97 %. The structure of SIPI-228 was elucidated by MS and NMR spectra. The results showed that its structure was identical to that of romidepsin.
作者 熊磊 张长清 胡海峰
机构地区 中国医药工业研究总院上海医药工业研究院
出处 《中国医药工业杂志》 CAS CSCD 北大核心 2016年第8期1005-1007,共3页 Chinese Journal of Pharmaceuticals
基金 国家“重大新药创制”重大专项(2014ZX09201001-005-001) 上海市自然科学基金(15ZR1440300)
关键词 SIPI-228 紫色色杆菌 罗米地辛 分离纯化 结构鉴定 SIPI-228 Chromobacterium violaceum romidepsin isolation and purification structure identification
分类号 TQ465 [化学工程—制药化工]
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参考文献6

  • 1Ito T, Ouchida M, Morimoto Y, et al. Significant growth suppression of synovial sarcomas by the histone deacetylase inhibitor FK228 in vitro and in vivo [J]. Cancer lett, 2005, 224(2):311 319.
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  • 3VanderMolen KM, McCulloch W, Pearce C J, et al. Romidepsin (Istodax, NSC 630176, FR901228, FK228, depsipeptide) : a natural product recently approved for cutaneous T-cell lymphoma [J]. JAntibiot (Tokyo), 2011, 64 (8) : 525--531.
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二级参考文献10

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中国医药工业杂志
2016年 第8期

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