摘要
建立了同时检测植物饮料中原百部碱、香豆素、黄连素、山道年、细辛脑、长叶薄荷酮和芦荟苷A7种植物毒素含量的高效液相色谱法。试样经HLB固相萃柱富集净化,甲醇洗脱,氮吹浓缩后用流动相起始梯度定容至1.0mL,经Welch Ultimate XB-C18色谱柱(4.6mm×150mm,5μm)分离,以甲醇-10mmol/L乙酸铵水溶液(pH=3)为流动相梯度洗脱,通过紫外检测器进行检测。7种植物毒素化合物在0.20~100.00mg/L范围内线性关系良好,相关系数均〉0.99,方法的检出限分别为0-2~6.5μg/kg,定量限为0.8~21.6μg/kg。分别对3种植物饮料样品进行加标回收率实验,回收率为67.1%~104.0%,相对标准偏差(n=6)为1.08%~5.12%。该方法操作简单,具有较高的准确度和精密度,适用于植物饮料中7种植物毒素含量的检测。
A new method was developed for the simultaneous detection of seven phytotoxin compounds (protostemonine, coumarin, berberine, san- tonin, β-asarone, R-(+)-pulegone, aloin A) in plant beverage by HPLC. The samples were purified with HLB solid phase extraction column, eluted by methanol, concentrated by nitrogen blowing, and separated by gradient elution in a Welch Ultimate XB-C18 column (4.6 mm×150 mm, 5 μm) with methanol-ammonium acetate (10 mmol/L, pH=3) as mobile phase (initial volume 1.0 ml), and then detected by UV detector. Under the optimal con- ditions, the calibration curves for the seven analytes were linear within the range of 0.20-100.0 mg/L and the correlation coefficients were higher than 0.99. The detection limits and quantitation limits of the method were in the range of 0.2-6.5 μg/kg and 0.8-21.6 μg/kg, respectively. The mean recov- eries for three sample matrixes were in the range of 67.1%-104.0%, with the relative standard deviations (n=6) ranged from 1.08% to 5.12%. This method was simple and accurate, which could be applied to the detection of seven phytotoxin compounds in plant beverage.
出处
《中国酿造》
CAS
北大核心
2016年第8期169-172,共4页
China Brewing
基金
国家质检总局科技计划项目(2014QK052
2015QK161)