高效液相色谱同时测定植物饮料中7种植物毒素

Simultaneous detection of seven phytotoxin compounds in plant beverage by HPLC

建立了同时检测植物饮料中原百部碱、香豆素、黄连素、山道年、细辛脑、长叶薄荷酮和芦荟苷A7种植物毒素含量的高效液相色谱法。试样经HLB固相萃柱富集净化，甲醇洗脱，氮吹浓缩后用流动相起始梯度定容至1．0mL，经Welch Ultimate XB-C18色谱柱（4．6mm×150mm，5μm）分离，以甲醇-10mmol／L乙酸铵水溶液（pH=3）为流动相梯度洗脱，通过紫外检测器进行检测。7种植物毒素化合物在0．20～100．00mg／L范围内线性关系良好，相关系数均〉0．99，方法的检出限分别为0-2～6．5μg／kg，定量限为0．8～21．6μg／kg。分别对3种植物饮料样品进行加标回收率实验，回收率为67．1％～104．0％，相对标准偏差（n=6）为1．08％~5．12％。该方法操作简单，具有较高的准确度和精密度，适用于植物饮料中7种植物毒素含量的检测。

A new method was developed for the simultaneous detection of seven phytotoxin compounds （protostemonine, coumarin, berberine, san- tonin, β-asarone, R-（＋）-pulegone, aloin A） in plant beverage by HPLC. The samples were purified with HLB solid phase extraction column, eluted by methanol, concentrated by nitrogen blowing, and separated by gradient elution in a Welch Ultimate XB-C18 column （4.6 mm×150 mm, 5 μm） with methanol-ammonium acetate （10 mmol/L, pH=3） as mobile phase （initial volume 1.0 ml）, and then detected by UV detector. Under the optimal con- ditions, the calibration curves for the seven analytes were linear within the range of 0.20-100.0 mg/L and the correlation coefficients were higher than 0.99. The detection limits and quantitation limits of the method were in the range of 0.2-6.5 μg/kg and 0.8-21.6 μg/kg, respectively. The mean recov- eries for three sample matrixes were in the range of 67.1%-104.0%, with the relative standard deviations （n=6） ranged from 1.08% to 5.12%. This method was simple and accurate, which could be applied to the detection of seven phytotoxin compounds in plant beverage.