摘要
目的:研究运动对去卵巢大鼠肝脏组织固醇调节元件结合蛋白-1c(SREBP-1c)表达的影响,探讨运动在绝经后肝脏脂肪变性中的作用机制。方法:SPF级9周龄SD雌性大鼠24只,按体重随机分为假手术组(SHAM,8只)、去卵巢组(OVX,8只)和去卵巢后运动组(OVX+EXE,8只)。12周中等强度跑台运动后,取大鼠肝脏组织,观察肝脏组织病理学变化,测定肝脏组织胆固醇和甘油三酯含量;免疫组织化学技术定性和半定量描述SREBP-1c蛋白表达;酶联免疫吸附测定法检测SREBP-1c蛋白表达。结果:12周中等强度运动后,OVX+EXE组与OVX组相比,体重明显下降,降幅10.7%(P<0.05);肝脏TC含量下降23.9%(P<0.05),TG含量下降23.4%(P<0.05);SREBP-1c阳性细胞数下降2.3倍(P<0.01);SREBP-1c蛋白表达下降8.5%(P<0.05)。结论:中等强度运动能够降低去卵巢大鼠体重,可能通过下调SREBP-1c蛋白表达,降低肝脏TC、TG含量,抑制肝脏细胞脂肪变性进程,有效改善肝脏脂肪代谢紊乱。
Objective ： The aim was to identify the effects of moderate exercise on the expression of sterol regulatory element-binding proteins- lc （SREBP- It） in the liver tissue of ovarieetomized rats, and to explore the relationship between the expression of SREBP- lc and hepatic steatosis. Methods ： The sample consisted of 24 female SD rats of SPF level that were 9 weeks old. They were randomly divided into three groups ： sedentary （SHAM, 8） , ovarieetomized （OVX, 8 ）, and ovarieetomized rats subjected to moderate exercise （OVX ＋ EXE, 8 ）. After moderate treadmill exercise for 12 weeks, laparotomy was carried out to collect part of the liver. Pathomorphology was observed. Liver cholesterol and triglyceride were measured. The exwession of SREBP-1c in the liver was detected by immunohistoehemistry and ELISA. Results：After 12 weeks, compared to OVX group, the body weight of OVX ＋ EXE group dropped 10.7%（P〈 0.05 ） ; liver cholesterol dropped 23.9% （ P〈0.05 ）, liver triglyeeride dropped 23.4% （ P〈0.05 ） ; the number of SREBP-1c positive cells dropped 2.3 times （ P〈 0.01 ） ;and the expression of SREBP-lc dropped 8.5% （P〈0.05）. Conclusions：Moderate exercise could lose body weight, possibly down-regulate the expression of SREBP-1c, reduce the liver TC and TG content, inhibit the progress of hepatic steatosis, improve effectively fat metabolism disorder in liver tissue of ovariectomized rat.
出处
《天津体育学院学报》
CAS
CSSCI
北大核心
2016年第3期259-263,共5页
Journal of Tianjin University of Sport
基金
河南省教育厅自然科学研究计划项目(项目编号:12A630130)
