用于检测生物硫醇的新型萘酰亚胺荧光探针的合成与应用被引量：2

The synthesis and application of a new type of naphthalimide fluorescent probe for biothiols determination

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摘要该文利用硫醇诱导的2,4-二硝基苯磺酰基基团的断裂反应生成具有供-吸电能力的荧光团,成功设计合成一种基于萘酰亚胺识别硫醇的荧光探针。相对于其他氨基酸,该探针在生理p H(7.40)值下,对于含硫醇的氨基酸具有高度的选择性和灵敏度。探针对硫醇具有显著的荧光增强响应,其荧光强度可以恢复197倍。此外,细胞内硫醇的荧光成像实验,证明该荧光探针具有潜在检测细胞内生物硫醇的能力。 The research used, 2,4-dinitrobenzenesulfonyl group cleavage reaction induced by thiols to generate fluorophore with power supply-sucking ability, and proposed and synthesized a kind of fluorescence probe that can identify biothiols via naphthalimides. Relative to other amino acids, the probe is under physiological pH value （7.40）, which has high sensitivity and selectivity on amino acids containing biothiols. The probe has a striking fluorescence enhancement effect on biothiols, which can recover fluorescence intensity to 197 times. Besides, fluorescence imaging test of biothiols in cells shows that the fluorescence probe has a potential ability of detecting biothiols in cells.

作者陈颂王静侯鹏刘磊王鑫

机构地区齐齐哈尔医学院药学院

出处《中国测试》 CAS 北大核心 2016年第8期64-67,共4页 China Measurement & Test

基金齐齐哈尔市科学技术计划项目(SFGG-201416)

关键词生物硫醇合成萘酰亚胺氨基酸断裂反应荧光探针 biothiols synthesis naphthalimide amino acids cleavage reaction fluorescence probe

分类号 Q523 [生物学—生物化学]

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