摘要
甘薯淀粉生产中会产生大量的废水,其中含有大量β-淀粉酶。采用超滤技术对废液中的β-淀粉酶进行纯化,研究了超滤系统几个主要参数对膜通量的影响。研究结果表明:操作压力0.14 MPa,操作温度20℃,操作时间40min,料液p H 7.0,截留分子量为50 k Da,在此条件下,酶活纯化倍数5.6倍,酶活回收率78%,纯化效果较佳。
Potato starch＇ production would produce large quantities of waste water,contains a lot of organic substances,such as β-amylase.β-Amylase from waste water were purified by ultra-filtration technology.How those major ultra-filtration-system parameters affecting the membrane flux were studied.It had showed that the ultra-filtration operating pressure was 0.14 MPa,operating temperature was 20 ℃,operating time was 40 min,p H of the feed solution was 7.0,and molecular weight cutoff was 50 k Da.Under these conditions,the degree of enzyme activity purification was 5.6-fold,the recovery of enzyme activity was 78%,the purification was better.
出处
《食品工业》
CAS
北大核心
2016年第8期53-56,共4页
The Food Industry
关键词
Β-淀粉酶
超滤
纯化
β-amylase
ultra-filtration
purification
