人参×西洋参F_1代及亲本种子愈伤组织氨基酸含量的比较

Comparison of Amino Acids on Seed Callus of F_1 Generation From Ginseng and American Ginseng and Their Parents

为了研究人参与西洋参杂交F_1代及其亲本种子愈伤组织的氨基酸含量,以"福星1号"人参和"中农洋参1号"西洋参为试材,采用高效离子交换色谱法,测定了F_1代和双亲本3种材料愈伤组织的氨基酸含量,并分析了氨基酸组成的差异性。结果表明:F_1代及亲本愈伤组织中均含有17种氨基酸,F_1代愈伤组织氨基酸含量相对较高,其次为母本、父本;3种材料均以谷氨酸、天冬氨酸含量最高,F_1代不同种类氨基酸和总氨基酸含量高于亲本,但不同种类氨基酸占总氨基酸的比重与亲本差异较小。

In order to determine the amino acid content of the callus,with the seeds in vitro of F_1 generation from P.ginsengC.A.Mey.and P.quinquefolium L.and their parents as material,the amino acids content and amino acid composition were determined and analyzed by the high performance ion exchange chromatography in three callus.The results showed that the callus of F_1 generation and their parents contained 17 kinds of amino acids.The amino acid of F_1 generation was higher than their patents＇,the lower were female parent,male parent.Glutamic acid and aspartic acid of the three materials were higher than others.The contents of types and total of amino acid of F_1 generation were higher than their parents＇.But the share of types and total of amino acid of F_1 generation and their parents was less than the difference.