小鼠小脑片层化过程中神经元迁移及reelin的可能调节作用

The neurondcell migration in the lamellar process of cerebellum of mouse and a possible contral role of reelin

目的：观察小鼠小脑皮质片层化过程细胞迁移,探讨片层化、细胞迁移和reelin蛋白之间的关系。方法：不同年龄的小鼠172只,应用免疫荧光、Nissl和HE染色法对小脑形态结构及片层化、细胞迁移情况进行观察。结果：（1）小脑片层化：E15-E16的后脑主要由神经上皮构成,P0时小脑皮质基本形成了外颗粒层、分子层、浦肯野细胞层和颗粒层,P5时浦肯野细胞层形成2~3层细胞,分子层仍有大量细胞。P20时基本形成了小脑的典型三层结构。（2）细胞迁移过程：浦肯野细胞大约在E18时开始迁移,至P7时基本完成迁移,胞体形成均一的1~2层细胞结构。同时,小年龄时NeuN阳性细胞主要见于内颗粒层和外颗粒层,P7后阳性细胞只定位在内颗粒层,迁移基本停止。（3）野生鼠（WT）和reeler小鼠比较：约P14时,WT小鼠小脑形成分子层、浦肯野细胞层及颗粒层。但是在reeler小鼠,小脑分叶不良,浦肯野细胞未迁移至目的地,排列紊乱;内颗粒层（GL）细胞分布松散、不均一。结论：在小鼠小脑的片层化形成过程中,Reelin蛋白可能参与神经细胞增殖和迁移的调控过程。

Objective： To observe cell migration in mice lamellar cerebellar cortex,and explore the relationship among lamellar process,cell migration and reelin protein. Methods： 172 mice at different ages were investigated Nissl and HE and immunofluorescence staining,to observe lamellar morphology and cell migration of cerebellum. Results：（ 1） Lamellar morphology of formation： E15-E16 hindbrain was mainly composed of neural epithelium. At P0,the granular layer,the molecular layer,Purkinje cells and granular layers formed were basically. At P5,the Purkinje cell layer formed 2 ~3 layers of cells,the molecular layer had a lot of cells. At P20,the cerebellum formed a typical three-layer structure： the molecular layer,Purkinje cell layer and granular layer.（ 2） Cell migration： about E18,the Purkinje cells began to migrate,at P7 Purkinje cells completed the migration,and sparsely arranged to form a homogeneous one to two layers of cells. Meanwhile,in younger mice most of Neun-positive cells were located in the inner and outer granular layers. At P7 positive cells only located in the inner granular layer,at this point the migration basically stopped.（ 3） Wild type（ WT）mouse and reeler mouse comparison： At P14,WT mouse cerebellum formed molecular layer,Purkinje cell layer and granular layer. But in the reeler mouse,cerebellar lobes were not clear,Purkinje cells did not migrate to the destination.Cells in inner granular layer（ GL） loosely distributed and were heterogeneous. Conclusion： In mouse cerebellum lamel-lar formation,reelin rotein might be involved in cell proliferation,differentiation and migration processes.