米诺环素对1-甲基-4-苯基-1,2,3,6-四氢吡啶诱导C57BL/6小鼠行为学损伤的抑制作用研究

Minocycline Inhibits MPTP-Induced the Behavior Damage in C57BL /6 Mice

目的探讨米诺环素对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导的C57BL/6小鼠的行为学损伤的保护作用及其机制。方法通过MPTP腹腔注射C57BL/6小鼠建立帕金森病亚急性模型。40只C57BL/6雄性小鼠随机分为对照(control)组,模型(MPTP)组,治疗(MPTP+Mino)组和米诺环素阴性对照(Mino)组,每组10只。分别采用游泳、爬杆和自主活动计数实验检测小鼠的运动行为能力。并采用HPLC测定中脑多巴胺(DA)、二羟苯乙酸(DOPAC)和高香草酸(HVA)的含量。结果治疗组游泳评分,爬杆评分和自主活动能力明显高于模型组(P<0.01)。且治疗组的中脑DA、DOPAC和HVA的含量明显高于模型组(P<0.01)。结论米诺环素对MPTP所致的小鼠运动功能损伤具有良好的保护作用,其机制可能是通过抑制MPTP所致的小鼠多巴胺神经元的损伤而起作用。

OBJECTIVE To investigate the effect of minoeycline on the behavior damage induced by 1-methyl-4-phenyl-1,2,3, 6-tetrahydropyridine（MPTP） in C57BL/6 mice. METHODS The PD models were formed with intraperitoneal injections of MPTP in C57BL/6 mice. Forty mice were randomly divided into 4 groups： control group, MPTP group, MPTP ＋ Mino group and Mino group（ 10 for each group）. The levels of midbrain DA, DOPAC and HVA were measured by HPLC after behavior tests（ swimming test, pole test and locomotor activity test ） were performed. RESULTS Swimming scores, climbing score and locomotor activity of MPTP ＋ Mino group were significantly higher than the MPTP group（ P 〈 0. 01 ）. And the content of midbrain DA, DOPAC and HVA of MPTP ＋ Mino group was significantly higher than the MPTP group （ P 〈 0. 01 ）. CONCLUSION Minocycline inhibits MPTP-induced damage of motor function in mice, the mechanism may be work via inhibiting MPTP-induced mice dopamine neuron damage.