红枣色素中活性成分含量与抗氧化活性的相关性

Correlation between active component contents and antioxidant activity of jujube pigment

为明确红枣色素抗氧化作用的物质基础,测定碱提、超声辅助碱提、酶-超声辅助碱提色素中总多酚、花色苷和总黄酮含量,采用还原能力、O-2·和DPPH·清除能力评价其抗氧化活性,并分析色素活性成分含量与抗氧化活性之间的关系。结果表明:不同方法提取色素活性成分含量存在显著差异(p<0.05),以碱提色素最高;不同方法提取色素均具有较强抗氧化活性,且清除O-2·、DPPH·能力差异显著(p<0.05);相关性研究显示总多酚、总黄酮含量与清除O-2·、DPPH·能力呈显著正相关(p<0.05),花色苷含量与清除DPPH·(r=0.874)能力呈明显正相关,表明多酚和黄酮可能是红枣色素抗氧化作用的主要物质基础。

In order to determine the material base of antioxidation effect of jujube pigment, the total polyphenols, anthocyanins and flavonoids content of the pigment respectively obtained by alkali extraction,ultrasound-assisted alkali extraction and enzyme-ultrasound assisted alkali extraction were measured. Antioxidant activities of the pigment were evaluated by three antioxidant methods, including reducing power, superoxide anion radical scavenging capacity and DPPH radical scavenging capacity, and the correlative relationships between the contents of active components and antioxidant activity in pigment were analyzed.The results showed that the contents of active components on different extraction methods had significantly difference with each other （p 〈 0.05） ,and the contents of active components by alkali extraction were highest.The pigments extracted by different methods had good antioxidant activities,and had a significant difference on scavenging superoxide anion radical and DPPH radical（p 〈0.05）.The results of correlation study showed that the correlations between the contents of total polyphenols,total flavonoids and superoxide anion radical, DPPH radical scavenging capacity were significant positive（p 〈0.05）.There also was an obviously positive correlation between the contents of anthocyanins and the scavenging capacity on DPPH radical（r =0.874）.The results indicated that polyphenols and flavonoids might be the main material base of antioxidation effect of jujube pigment.