基于ISSR分子标记分析云南蚕区44份家蚕品种资源的亲缘关系

Genetic Relationship Analysis of 44 Silkworm Germplasm Resources in Yunnan Province Based on ISSR Molecular Makers

应用ISSR分子标记技术分析云南蚕区保存的44份家蚕品种资源的亲缘关系,为家蚕种质资源的合理保存、利用及育种亲本选择提供依据。以筛选的13条ISSR引物从44个家蚕品种的蛹基因组DNA中扩增得到116个条带,其中多态性条带98条,多态性比率为84.5%,表明44个家蚕品种间具有丰富的遗传多样性。44个家蚕品种间的遗传相似系数为0.517 2~0.905 2,平均遗传相似系数为0.711 2。基于品种间ISSR分子标记的遗传相似系数,采用UPGMA法进行聚类分析,供试品种首先按中系和日系形成2大类群,在遗传相似系数0.69处,2大类群又各自再分成2个组群。4个组群中,均是育种材料亲缘关系较近的品种聚在同一组群内。ISSR分子标记结果较好地揭示了云南蚕区44份家蚕品种资源之间的遗传差异和亲缘关系。

In this study,the genetic relationship of 44 silkworm（ Bombyx mori） variety resouces conserved in Yunnan Province was investigated by means of ISSR molecular marking technology to provide the basis for the rational preservation,utilization and selection of parental silkworm varieties of silkworm germplasm resources. Totally 116 bands were amplified from pupal genomic DNA of 44 silkworm varieties using 13 ISSR primers and there were 98 polymorphic bands with a percentage of polymorphic band of 84. 5%,implying that there was rich genetic diversity among the tested 44 germplasm resources of silkworm. Genetic similarity coefficients between the 44 silkworm varieties ranged from 0. 517 2 to0. 905 2,with an average of 0. 711 2. The genetic relationship between these silkworm varieties was analyzed with UPGMA method based on their genetic similarity coefficients. The result showed that the tested silkworm varieties were firstly clustered into Chinese and Japanese strain silkworm varieties,and then further divided into 2 subgroups respectively at genetic similarity coefficient of 0. 69 and the silkworm varieties with close genetic relationship were present in the same group. The results of ISSR molecular marking revealed the genetic diversity and genetic relationship of the tested 44 silkworm germplasm resources in Yunnan Province.