丙酮酸激酶在大肠癌组织中的表达水平研究

Study on the expression of PKM levels in colorectal cancer

目的研究丙酮酸激酶M1型(PKM1)和丙酮酸激酶M2型(PKM2)在大肠癌临床样本中的表达水平,探讨其与大肠癌发生发展的相关性。方法分别采用Western blot法检测大肠癌临床标本和新鲜大肠癌组织及癌旁组织中PKM1和PKM2蛋白表达水平;分析PKM1、PKM2蛋白表达与大肠癌发生发展的相关性。结果 Western blot和免疫组化结果均显示:与癌旁组织相比,癌组织中PKM1的表达水平明显降低(P<0.05),PKM2的表达水平明显增高(P<0.05)。相关分析结果表明,PKM1与大肠癌患者年龄,性别,分期均无相关性,PKM2与病理分期密切相关(P<0.05)。结论大肠癌组织中PKM1呈现低表达,PKM2高表达。且PKM2与大肠癌患者的病理分期成正相关。

Objective To study the expression level of PKM1 and PKM2 in colorectal cancer tissue and explore the relationship between colorectal cancer progression and their expression level. Methods The PKM1 and PKM2 protein expression levels in colorectal cancer tissues and adjacent tissues to carcinoma were determined by Western blot and Immunohistochemisty method, and the relationship with colorectal cancer were discussed.Results Western blot and Immunohistochemisty results all showed that,compared with adjacent noncancerous,the protein level of PKM1 decreases significantly in cancer tissues（ P〈0. 05）,while the protein level of PKM2 increases significantly in cancer tissues（ P〈0. 05）. The protein level of PKM1 is not relative with age,sex and stage of patients,but the protein level of PKM2 is relative with p TNM（ P〈0. 05）. Conclusion Colorectal cancer tissues with low level of PKM1 and high level of PKM2,and PKM2 was related with TNM of patients.