壳聚糖纳米银复合物基温敏水凝胶的抑菌性及细胞相容性研究

Antibacterial activity and cytocompatibility of chitosan-nano-silver complex thermosensitive hydrogel

目的以实验室自制的壳聚糖纳米银复合物基温敏水凝胶为材料,探讨其抑菌性及细胞相容性。方法设置4组:A(含纳米银1×10-5的壳聚糖纳米银复合物基温敏水凝胶),B(含纳米银5×10-6的壳聚糖纳米银复合物基温敏水凝胶),C(含纳米银2×10-6的壳聚糖纳米银复合物基温敏水凝胶),D(壳聚糖温敏水凝胶,不含纳米银)。通过抑菌圈法测定4种样品对6种革兰阴性菌,1种革兰阳性菌,3种真菌的抑菌作用;以SRB法测定其浸提液的细胞相容性。结果随着壳聚糖纳米银复合物基温敏水凝胶中纳米银含量增加,抑菌作用增强,壳聚糖纳米银复合物基温敏水凝胶的抑菌作用优于壳聚糖温敏水凝胶;其浸提液无细胞毒性,具有良好的细胞相容性。结论壳聚糖纳米银复合物基温敏水凝胶有望作为新型创伤敷料。

Objective To explore the antibacterial activity and cytocompatibility of chitosan-nano-silver complex thermosensitive hydrogel.Methods There were 4 groups： group A（ containing 1 ×10^-5chitosan-nano-silver complex thermosensitive hydrogel）,group B（ containing 5 ×10^-6chitosan-nano-silver complex thermosensitive hydrogel）,group C（ containing 2 ×10^-6chitosan-nano-silver complex thermosensitive hydrogel）,group D（ chitosan thermosensitive hydrogel）. The antibacterial activity of the samples against six kinds of gram-negative bacteria,one kind of gram-positive bacterium,three kinds of fungi were measured by bacteriostatic circle. The cytocompatibility of the extraction to NIH-3T3 cells was studied by SRB.Results The antibacterial activity enhanced with the increasing of nano silver concentration in chitosan-nano-silver complex thermosensitive hydrogel,whose antibacterial activity was better than chitosan thermosensitive hydrogel; its extraction has no cytotoxicity,thus showed good cytocompatibility.Conclusion The chitosan-nano-silver complex thermosensitive hydrogel is a potential novel wound dressing.