纳米山药多糖对4种肿瘤细胞的作用

Effect of Nano Chinese Yam Polysaccharide on 4 Kinds of Tumor Cells

目的研究纳米山药多糖的抗肿瘤活性,并对其作用机制进行探究。方法细胞增殖/毒性检测试剂盒(CCK-8试剂盒)测定各给药组对肿瘤细胞抑制率的影响;Western blotting检测不同给药组给药48 h后对4种肿瘤细胞凋亡相关蛋白caspase-3、caspase-8表达情况的影响;细胞划痕实验检测各给药组对肿瘤细胞迁移能力的影响。结果 CCK-8实验表明,与对照组相比,纳米山药多糖高剂量组(80 ng·m L?1)、氟尿嘧啶组(80 ng·m L?1)能够明显抑制肿瘤细胞增殖(P<0.05);Western blotting实验显示纳米山药多糖高剂量组(80 ng·m L?1)与氟尿嘧啶组(80 ng·m L?1)肿瘤细胞凋亡蛋白caspase-3、caspase-8条带灰度降低,蛋白含量表达与对照组比较明显减少(P<0.05);细胞划痕实验表明纳米山药多糖作用于4种肿瘤细胞48 h后,细胞愈合率均有不同程度的下降(P<0.05)。结论纳米山药多糖剂量的升高,促进肿瘤细胞凋亡蛋白caspase-3和caspase-8蛋白酶原的活化,酶原降解增多,抑制肿瘤细胞生长,促进肿瘤细胞凋亡,降低细胞划痕愈合率,具有抗肿瘤活性。

OBJECTIVE To study anti-tumor activity of nano chinese yam polysaccharide and to explore the mechanism of its action. METHODS Cell proliferation/toxicity detection kit（CCK-8 kit） was used to determine inhibition rate to tumor cells of each drug administration group; Western blotting was used to test the effect of each drug administration group on the expression of tumor cell apoptosis related protein caspase-3, caspase-8 after 48 h. Effect of each drug administration group on the ability of tumor cell migration was tested by tumor cell scratch experiment. RESULTS CCK-8 experiment showed that compared with normal control group, high dose group of nano chinese yam polysaccharide（80 ng·m L^-1） and fluorouracil group（80 ng·m L^-1） could obviously inhibit tumor cell proliferation（P〈0.05）; Western blotting experiment showed that the tumor cell apoptosis protein caspase-3, caspase-8 of nano chinese yam polysaccharide group（80 ng·m L^-1） and fluorouracil group（80 ng·m L^-1） had lower band grayscale, protein expression were significantly increased compared with the normal group（P〈0.05）; Cell scratch experiment showed that healing rate of 4 kinds of tumor cells treated with nano chinese yam polysaccharide all had varying degrees of decline（P〈0.05） after 48 h. CONCLUSION The increase of the dosage of nano chinese yam polysaccharide can promote the original tumor cell apoptosis caspase-3 and caspase-8 protease activation, increase the degradation of zymogen, inhibit the growth of tumor cells, promote tumor cell apoptosis and reduce cell scratch healing rate, nano chinese yam polysaccharide has anti-tumor activity.