灵芝中3种三萜酸的含量测定与特征图谱研究

Determination of Three Triterpenoid Acid in Ganoderma and Its Characteristic Chromatograms

目的建立测定灵芝中灵芝三萜含量及特征图谱的方法。方法采用高效液相色谱法,色谱柱为Agilent-SB C18（250 mm×4.6 mm,5μm）,流动相为乙腈（A）-0.1%冰醋酸（B）（30∶70）,梯度洗脱（0~20 min A 30%→40%）,流速为1.0 m L·min^-1,柱温30℃,检测波长为254 nm。利用相似度评价系统进行指纹图谱相似度计算。结果灵芝中灵芝三萜类成分较高,灵芝指纹图谱相似度达到0.93以上。结论灵芝中灵芝三萜类成分含量高,特征图谱方法稳定、重现性好,可用于灵芝的质量控制。

OBJECTIVE To establish determination method for triterpenoid content in Ganoderma and study its of characteristic chromatograms. METHODS HPLC was applied with the chromatographic condition as follows： the chromatographic column was Agilent Zorbax SB-C18（250 mm×4.6 mm, 5 μm） at 30 ℃; mobile phase was acetonitrile –0.1% glacial acetic acid（30∶70）; flow rate was 1.0 m L·min^-1, the detection wavelength was 254 nm. Using the similarity evaluation system for fingerprint similarity calculation. RESULTS Ganoderma triterpene component was high, Ganoderma fingerprint similarity was above 0.93. CONCLUSION Ganoderma triterpene component content in Ganoderma is high, the characteristic chromatograms method is stable, good reproducibility, and can be used for quality control of Ganoderma.