基于灰色关联度分析法对半夏及其炮制品总有机酸止咳作用的谱-效关系研究

Spectrum-effect relationship of antitussive effect by total organic acids in crude and four processed Pinellia ternate based on gray relative analysis method

目的研究半夏及其炮制品清半夏、姜半夏、法半夏和京半夏总有机酸HPLC指纹图谱与止咳作用的相关性,建立半夏及其炮制品总有机酸与止咳作用的谱-效关系,以阐明止咳作用的物质基础。方法采用灰色关联度分析法,对半夏及其炮制品总有机酸HPLC指纹图谱的各共有峰峰面积与止咳的3个评价指标(咳嗽潜伏期、3 min内咳嗽次数及抑咳率)进行相关性分析,建立半夏及其炮制品总有机酸止咳作用的谱-效关系。结果半夏及其炮制品总有机酸对浓氨水致小鼠咳嗽有显著的抑制作用,与半夏止咳功效一致。在半夏及其炮制品总有机酸HPLC指纹图谱中,4、6(琥珀酸)、12号色谱峰所代表的化学成分与止咳作用具有较强的相关性,对止咳作用贡献较大,特别是4号色谱峰,峰面积大、关联度强。结论半夏及其炮制品总有机酸HPLC指纹图谱与其止咳作用具有相关性。通过谱-效关系研究,阐明了半夏及其炮制品止咳作用的物质基础,为其质量控制与评价提供了理论依据。

Objective To investigate the HPLC fingerprint of the total organic acids in Pinelliae Rhizoma（the rhizomes of Pinelliae ternate）, Pinelliae Rhizoma Praeparatum cum Alumine, Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine, Pinelliae Rhizoma Praeparatum and Pinelliae Rhizoma Praeparatum cum Glycyrrhizae et Alumine, and the its relationship with antitussive effect, and to establish the spectrum-effect relationship of the total organic acids in crude and processed P. ternate and antitussive effect, so as to elucidate their material basis of antitussive effect. Methods The gray relative analysis method was used to make correspond analysis of the HPLC fingerprints of total organic acids in Pinelliae Rhizoma and processed products related with three antitussive indexes（the latent period, coughing times in 3 min, and inhibitatory rate）, establishing the spectrum-effect relationship between them. Results The total organic acids of crude Pinelliae Rhizoma and its processed products showed the antitussive activity against the ammonium hydroxide-induced mice cough, consist with arresting cough of Pinelliae Rhizoma. The HPLC data demonstrated that the chemical composition represented by peak 4, 6（succinic acid）, and 12 had strong relevancy with anti-tussive activity and made a great contribution to this activity, especially peak 4, which had bigger area and stronger relevancy. Conclusion This paper reveals that there exists the correlations between the HPLC fingerprints of total organic acids and antitussive effect of crude and processed Pinelliae Rhizoma, elucidating the material basis of antitussive effects in crude and processed P. ternate by the spectrum-effect study, and could provide a theory basis for the quality control and analysis of Pinelliae Rhizoma.