番茄红素联合跑台运动对去卵巢大鼠骨质疏松模型的影响

The effect of lycopene in combination with treadmill exercise in an osteoporosis rat model

目的通过观察番茄红素和跑台运动对去卵巢大鼠骨代谢指标的影响,探究番茄红素联合运动对去势大鼠骨代谢的作用机制。方法 6月龄雌性Wistar大鼠,按体质量随机分为6组:假手术组(Sham组)、去卵巢组(OVX组)、去卵巢+运动组(OVX+E组)、去卵巢+番茄红素组(OVX+L组)、去卵巢+运动+番茄红素组(OVX+E+L组)、去卵巢+阿伦磷酸钠组(OVX+AL组)。在12周后取材进行相关指标测试。结果 (1)单纯性的跑台运动能降低去势大鼠脂肪量,提高骨小梁数目的同时提高骨形成标志物骨钙素(osteocalcin,OC)水平,促进骨形成。(2)单纯性的番茄红素疗法能提高去势大鼠的子宫重量、血清甲状腺激素水平,提高相对骨体积、骨小梁厚度和骨内膜骨形成率,增强骨密度的同时降低骨代谢标志物Ⅰ型胶原氨基末端交联肽(NTx)水平,抑制骨吸收。(3)运动和药物交互作用能降低去势大鼠的脂肪百分比,提高血清雌二醇水平,降低尿8-OHd G和尿脱氧吡啶酚(DPD)水平。改善去势大鼠骨小梁结构参数,提高骨外膜骨形成率、骨强度和抗性变能力,改善全身骨密度。结论 (1)适度的跑台运动可以防止去势大鼠骨丢失,改善去势大鼠骨健康。(2)12周的番茄红素对去势大鼠骨代谢的治疗作用与阿仑膦酸钠效果类似,提高骨强度,降低体内氧化还原反应、加强抗氧化系统功能,维持骨代谢平衡。(3)番茄红素和运动表现出良好的协同作用,对去势大鼠骨重建具有积极的作用。

Objective To study the effect of lycopene and exercise on bone metabolism in ovariectomized rats,and to explore the relevant mechanism. Methods 6 month old female Wistar rats,were random divided into 6 groups stratified by body mass： sham group（ sham）,OVX group（ OVX group）,OVX ＋ E group（ OVX ＋ Exercise group）,OVX ＋ L group（ OVX ＋ lycopene group）,OVX ＋ E ＋ L group（ OVX ＋ Exercise ＋ lycopene group）,OVX ＋ A group（ ovariectomy ＋ alendronate group）. After 12 weeks,the relevant indicators were tested. Results（ 1） Treadmill exercise could decrease fat body mass and improve OC（ bone formation marker） levels in ovariectomized rats,as well as improve trabecular number and inhibit bone resorption.（ 2） Lycopene monotherapy could improve uterine weight and serum thyroid hormone level,and decrease the level of NTX in ovariectomized rats. It also increased the relative bone volume,trabecular thickness and bone formation rate,and promoted bone formation.（ 3） Exercise in combination with drug treatment could decrease the percentage of body fat in ovariectomized rats,increase the level of serum estradiol,and reduce the level of 8-OHd G and DPD in urine. It also improved the structural parameters of trabecular bone,the rate of bone formation,bone strength and resistance,and whole body bone mineral density in ovariectomized rats. Conclusion（ 1）Moderate treadmill exercise can prevent bone loss and improve bone health in ovariectomized rats.（ 2） The effects of lycopene on bone metabolism in ovariectomized rats was similar to that of alendronate after 12 weeks,including increased bone strength,reduced oxidation,enhanced function of the antioxidant system and maintenance of bone metabolism balance.（ 3） Lycopene and exercise showed great synergistic effect,and had positive effects on bone remodeling in ovariectomized rats.