氦氖激光对退变颞下颌关节BMP-2表达的影响

Effects of He-Ne laser on BMP-2 expression in degenerated temporomandibular joint of rabbits

目的:探讨氦氖激光对退变颞下颌关节细胞外基质调节因子BMP-2表达的影响,明确氦氖激光干预颞下颌关节退变的作用机制,为临床运用氦氖激光治疗颞下颌关节骨关节病提供依据。方法:将骨骼发育成熟的健康成年雄性新西兰大白兔40只随机分为正常组、假模型组、模型组和治疗组,每组10只,组内又分为造模后第1d、11d两个取材时间点。建立颞下颌关节骨关节病动物模型并行CT检查证实造模成功后纳入。治疗组采用氦氖激光照射患侧颊车、上关、下关、听宫、翳风穴,5min/穴/d,共10d;假模型组仅作关节囊切开后即缝合组织。各组于不同时间点取出颞下颌关节组织,采用Western Blot方法检测BMP-2的表达水平。结果:Western Blot检测发现正常组及假模型组不同时间点之间BMP-2蛋白表达差异无统计学意义;模型组第11dBMP-2表达明显降低;氦氖激光干预10d后BMP-2表达较第1d及模型组明显增加(P<0.05)。结论:氦氖激光治疗可上调退变颞下颌关节细胞外基质调节因子BMP-2蛋白表达,促进细胞外基质合成,从而防治颞下颌关节骨关节病。

Objective： To observe the effects of He-Ne laser on the expression of BMP-2 in degenerated tem- poromandibular joint of rabbits, explore its protective role and mechanism in relieving temporomandibular joint de- generation and provide the basis for clinical application of He-Ne laser in the treatment of temporomandibular joint osteoarthritis. Methods= Forty New Zealand white rabbits were randomly divided into 4 groups： normal group, sham model group, model group and treatment group, 10 in each group. The specimens were harvested at 1st, and llth day after modeling. The animal model of temporomandibular ioint disease was established by the methods of ChangJia. He-Ne laser was rayed at Jiache, Shangguan, Xiaguan, Tinggong, Yifeng points for 10 days in the treat- ment group. In the sham model group, the joint capsule was incised and sutured. The animals were killed at 1st, and llth day and the protein expression of BMP-2 in temporomandibular joints was examined by Western blotting. Results= BMP-2 protein expression had no statistically significant difference between different time points in the nor- mal group and the sham model group, hut that was up-regulated in the treatment group after He-Ne laser treatment for 10 days. Conelusions： He-Ne laser on acupoints may protect the degenerated temporomandibular joint through up-regulating the expression of BMP-2.