胺基纳米磁珠提取血液中3种毒品

Using Amino-modified Nano-magnetic Beads to Extract Three Drugs from Human Blood

目的 建立一种利用胺基纳米磁珠提取血液中甲基苯丙胺、吗啡和氯胺酮的新方法。方法 将二乙胺与磁粉材料结合制成胺基纳米磁珠,在p H8.5的缓冲溶液中进行激活和洗脱。用其提取血液中甲基苯丙胺、吗啡和氯胺酮。结果 经GC-MS检测,胺基纳米磁珠可以有效富集血液中的甲基苯丙胺、吗啡和氯胺酮,回收率均高于70%,富集倍数均大于30倍。对提取强度、p H值、洗脱液用量、标准曲线、检测限、以及日间精密度与日内精密度等影响因素的实验结果表明：当采用漩涡方式、p H大于7、洗脱液用量为80μL时,提取率较高。结论 在GC-MS分析中,甲基苯丙胺、吗啡和氯胺酮血药浓度在0.5-10μg/m L范围内呈线性关系,r分别是0.9826、0.9526和0.9914,最低检出限分别是0.062、0.093和0.041μg/m L。提取甲基苯丙胺、吗啡和氯胺酮的日内精密度和日间精密度均小于6.0%。该方法富集效率高,吸附所需时间短,能有效提高血液中常见毒品的检测灵敏度。

A new method has been developed with amino-modiifed nano-magnetic beads to extract methamphetamine, morphine and ketamine from human blood. The amino nano-magnetic beads, generated through combination of diethylamine with magnetic powder, were used to extract drugs from human blood. The GC/MS results showed that the produced beads effectively enriched the drugs in blood, with a recovery rate higher than 70 %. On average, the enrichment ratio was greater than 1：30. All of the important parameters were studied at the extraction strength, pH value, eluent consumption, standard curve, detection limit as well as the intra- and inter-day precisions, indicating that a higher extraction can be reached upon the employment of vortex, pH greater than 7 and the consumed elution at 80 μL. For GC/MS analysis, the linear range was from 0.5 to 10.0 μg/mL, correlation coefifcients （r） were 0.9826,0.9526 and 0.9914, and the detective limits were individually 0.062, 0.093 and 0.041μg/mL, respectively. The intra- and inter-day precisions were both less than 6.0 %. This method is of high enrichment efifciency and short adsorption time, capable of improving the detection sensitivity of drugs in blood.