三角孔多孔板水力空化杀灭大肠杆菌的研究

Experimental study on Escherichia coli killed by hydrodynamic cavitation behind triangular multi-orifice plates

实验基于自主研发的新型多孔板水力空化空蚀反应装置,选取病原微生物中较常见的大肠杆菌为指示菌,用压力数据采集系统测定了空化空蚀工作段的压力,用琼脂平板计数法检测空化空蚀作用前后大肠杆菌的菌落数,同时用生物显微镜观察其形态变化。通过不同孔口流速、不同菌液的初始浓度和不同几何参数的多孔板对大肠杆菌进行空化空蚀实验,分析了空化数、孔口流速、大肠杆菌初始浓度、孔口数量、孔口大小和孔口排列方式对大肠杆菌杀灭率的影响,阐述了水力空化空蚀作用对大肠杆菌的杀灭机理。

In this study, experimental tests on hydrodynamic cavitation for disinfection were conducted on a reactor of multi-orifice plates that was independently developed in the Hydraulics Laboratory at Zhejiang University of Technology, and Escherichia coli that is pathogenic microorganism, was chosen as indicator bacteria. We measured time-average pressures of the flow behind the multi-orifice plates using a pressure data acquisition system, and collected water samples in the process of cavitation. A number of Escherichia coli bacteria were cultured in an agar plate, and changes in morphology were observed using a biological microscope. This paper focuses on an analysis of the disinfection of Escherichia coli impacted by cavitation number, orifice velocity, initial concentrations, number of orifice, arrangement of orifice and size of orifice, along with a brief illustration of the bactericidal mechanism of Escherichia coli under the impact of hydrodynamic cavitation.