MALDI-TOF-IMS分析DAI大鼠脑组织内差异蛋白的分布

Analysis of Differentially Expressed Proteins Distribution in the Rat Brains with DAI by MALDI-TOF-IMS

目的 基于基质辅助激光解吸/电离-飞行时间质谱成像（matrix assisted laser desorption/ionizationtime of flight imaging mass spectrometry,MALDI-TOF-IMS）技术建立弥漫性轴索损伤（diffuse axonal injury,DAI）大鼠脑组织内蛋白差异化表达的质谱成像方法。方法 应用AutoflexⅢ MALDI-TOF质谱仪在质荷比1 000~20 000范围内对DAI组和对照组脑组织进行质谱扫描,利用Clin Pro Tool 2.2软件对两组数据进行统计学分析,挑选出存在表达差异的蛋白质分子进行质谱成像,观察不同质荷比的蛋白质分子在脑组织中的分布情况。结果 质荷比为4 963、5 634、6 253、6 714及7 532的5个蛋白质分子在DAI大鼠脑组织内表达量存在差异。结论 MALDI-TOF-IMS可用于大鼠DAI后脑组织内差异蛋白的研究,初步建立了质谱成像技术检测DAI大鼠脑组织中差异蛋白分布的分析方法。

Objective To establish the imaging mass spectrometry for analysis of differentially expressed proteins distribution in the rat brains with diffuse axonal injury （DAI） based on matrix assisted laser desorption/ionization-time of flight imaging mass spectrometry （MALDI-TOF-IMS）. Methods MALDI- TOF-IMS scanning were conducted on the brains of DAI group and control group in the m/z range of 1 000 to 20000 using AutoflexIII MALDI-TOF spectrometer. Clin_ProTool 2.2 software was used for sta- tistical analysis on the data of two groups, and then the differentially expressed proteins were picked out to conduct imaging. The distribution of the proteins with different m/z in the rat brains was observed. Results Five proteins with different m/z, including 4 963, 5 634, 6 253, 6 714 and 7 532, differentially expressed in the rat brains with DAI. Conclusion MALDI-TOF-IMS can be used for studying the differen- tially expressed proteins in rat brains with DAI and the analysis method is established for exploring the distribution of differentially expressed proteins in the rat brains with DAI using imaging mass spectrometry.