应用微生物菌群鉴定阴道分泌液

Identification of Vaginal Fluid Using Microbial Signatures

目的探究特异性存在于阴道分泌液中的微生物菌群。方法收集阴道分泌液16份、唾液样本16份、粪便样本16份、精液样本8份、外周血样本8份、尿液样本5份及鼻腔分泌液样本4份,对卷曲乳杆菌、加氏乳杆菌、詹氏乳杆菌、惰性乳杆菌和阿托波氏菌的16S r RNA基因进行扩增,PCR产物采用3130xl型遗传分析仪进行检测。结果卷曲乳杆菌、加氏乳杆菌、詹氏乳杆菌、惰性乳杆菌和阿托波氏菌在阴道分泌液中的检出份数分别为15、5、8、14和3份。卷曲乳杆菌和詹氏乳杆菌特异性存在于阴道分泌液中。结论检测卷曲乳杆菌和詹氏乳杆菌对鉴定阴道分泌液具有潜在的应用价值。

Objective To investigate the specific microbial signatures in vaginal fluid. Methods Vaginal fluid （16 samples）, saliva （16 samples）, feces （16 samples）, semen （8 samples）, peripheral blood （8 samples）, urine （5 samples）, and nasal secretion （4 samples） were collected respectively. The 16S rRNA genes of Laetobacillus crispatus, Laetobaeillns gasseri, Laetobaeillns jensenii, Laetobaeillus iners, and Atopobiura vaginae were amplified. PCR production was detected via a 3130xl Genetic Analyzer. Results The detected number of Laetobaeillns crispatus, Laetobaeillns gasseri, Laetobaeillns jensenii, Laetobaeillns iners, and Atopobium vaginae were 15, 5, 8, 14, and 3 in all vaginal fluid samples, respectively. Laeto- bacillus crispatns and Laetobaeillns jensenii existed specifically in vaginal fluid. Conclusion There is a potential application value to detect Laetobaeillns crispatus and Lactobaeillus jensenii for the identification of vaginal fluid.