期刊文献+

广东地区汉族人群VEGF基因5′端SNP位点遗传多态性 被引量:1

Genetic Polymorphisms of SNP Located in the 5′ Region of VEGF Gene in Han Population in Guangdong
下载PDF
导出
摘要 目的 调查广东地区汉族人群VEGF基因5′端SNP位点的遗传多态性,为法医学应用及群体遗传学研究提供基础数据。方法 DNA微测序技术SNaPshot分析184例广东地区无关个体VEGF基因5′端4个SNP位点(rs699947、rs1570360、rs833061、rs2010963)的遗传多态性。应用PowerMarker v3.25软件进行统计分析。结果 184例广东地区汉族无关个体VEGF基因5′端4个SNP位点基因分布均符合Hardy-Weinberg平衡(P〉0.05),每个位点均检出3种基因型。rs833061和rs699947位点紧密连锁。共检出6种单倍型,其中C-G-T-C、C-G-T-G、A-A-C-G、A-G-C-G频率均〉10%,为主要单倍型。rs699947、rs833061、rs2010963位点的个人识别率为0.583~0.634,非父排除率为0.133~0.144;4个SNP构成单倍型的个人识别率为0.868,非父排除率为0.438。结论 广东地区汉族人群VEGF基因5′端序列呈现出高度遗传多态性,可作为个人识别和亲权鉴定的遗传学指标,同时可用于相关疾病关联分析。 Objective To investigate the genetic polymorphism of SNP located in the 5' region of the vascular endothelial growth factor (VEGF) gene in Han population in Guangdong and provide basic data for forensic application and population genetics research. Methods The genetic polymorphisms of 4 SNP loci (rs699947, rs1570360, rs833061, rs2010963) within 5' region of VEGF gene of 184 unrelated indi- viduals in Han population in Guangdong were analyzed by DNA micro sequencing technology SNaPshot. The statistical analysis was carried out by PowerMarker v3.25 software. Results The genotype distribu- tions of the 4 SNP loci within 5' region of VEGF gene of 184 unrelated individuals in Han population in Guangdong were in accordance with Hardy-Weinberg equilibrium (/'〉0.05) and 3 kinds of genotypes were detected from each loci. There was high linkage disequilibrium between the rs833061 and rs699947 SNP loci. Six haplotypes were observed, while the frequency of C-G-T-C, C-G-T-G, A-A-C-G and A-G-C-G were more than 10%, which were the main haplotypes. The discrimination probabilities (DP) of rs699947, rs833061, and rs2010963 loci were between 0.583 and 0.634, with the power of exclusion (PE) between 0.133 and 0.144. The DP and PE of haplotypes of 4 SNP were 0.868 and 0.438, respec- tively. Conclusion There are great polymorphisms in the 5' region of VEGF gene in Han population in Guangdong, which could be used as genetic indexes for individual identification and paternity testing, as well as association analysis of the related diseases.
出处 《法医学杂志》 CAS CSCD 2016年第4期257-260,共4页 Journal of Forensic Medicine
基金 上海市法医学重点实验室开放课题资助项目(KF1305) 广东省省级科技计划项目(2013B022000023)
关键词 法医遗传学 多态性 单核苷酸 血管内皮生长因子 汉族 forensic genetics polymorphism, single nucleotide vascular endothelial growth factor Hart nationality
  • 相关文献

参考文献8

  • 1Pang L, Wei Z, Li O, et al. An increase in vascular endothelial growth factor (VEGF) and VEGF soluble receptor-1 (sFlt-1) are associated with early recurrent spontaneous abortion[J]. Plos One, 2013,8 (9) : e75759.
  • 2Samli H, Demir BC, Ozgoz A, et al. Vascular en- dothelial growth factor gene 1154 G/A, 2578 C/A, 460 C/T, 936 C/T polymorphisms and association with recurrent pregnancy losses[J]. Genet Mol Res,2012, 11 (4) :4739-4745.
  • 3Lash GE, Innes BA, Drury JA, et oi. Localization of angiogenic growth factors and their receptors in the human endometrium throughout the menstrual cycleand in recurrent miscarriage[J]. Hum Reprod,2012, 27( 1 ) ~ 183-195.
  • 4李珠玉,游泽山,苏秀梅,张秀明,李再仪,吴再归,江丽.复发性流产与夫妻双方绒毛血管内皮生长因子基因多态性的关系[J].中山大学学报(医学科学版),2011,32(1):85-89. 被引量:2
  • 5Bayhan Z, Simsek T, Ergul E, et ol. Serum cytokine levels in patients with colorectal cancers according to tumor stages and VEGF gene polymorphism[J]. Hepato- gastroenterology, 2014,61 ( 135 ) ~ 1889-1894.
  • 6Nei M. Estimation of average heterozygosity and ge- netic distance from a small number of individuals[J]. Genetics, 1978,89(3 ) :583-590.
  • 7Prakash S, Agrawal S, Kumar S, et O1. Impact of vascular endothelial growth factor single nucleotide polymorphism association on acute renal allograft re- j ection[J]. Nephron, 2015,129 (2) : 91-96.
  • 8Peng ML, Tsai YY, Tung JN, et al. Vascular endothe- lial growth factor gene polymorphism and protein ex- pression in the pathogenesis of pterygium[J]. Br J Oph- thalmol, 2014,98 (4) :556-561.

二级参考文献13

  • 1Raj R,Lesley R.Recurrent miscarriage[J].Lancet,2006,368(8):601-611.
  • 2Burton GJ,Charnock DS,Jaunianx E.Regulation of vascular growth and function in the human placenta[J].Reproduction,2009,138(6):895-902.
  • 3Arroyo JA,Winn VD.Vasculogenesis and angiogenesis in the IUGR placenta[J].Semin Perinatol,2008,32(3):172-177.
  • 4Mohammadi M,Bazrafshani MR,Day PJ,et al.Vascular endothelial growth factor production is regulated by gene polymorphisms[J].Iran J Immunol,2009,6(3):119-129.
  • 5Papazoglou D,Galazios G,Papatheodorou K,et al.Vascular endothelial growth factor gene polymorphisms and idiopathic recurrent pregnancy loss[J].Fertil Steril,2005,83(4):959-963.
  • 6Coulam CB,Jeyendran RS.Vascular Endothelial Growth Factor Gene Polymorphisms and Recurrent Pregnancy Loss[J].Am J Reprod Immunol,2008,59(4):301-305.
  • 7Lee HH,Hong SH,Shin SJ.Association study of vascular endothelial growth factor polymorphisms with the risk of recurrent spontaneous abortion[J].Fertil Steril,2010,93(4):1244-1247.
  • 8Carmeliet P,Ferreira V,Breier G,et al.Abnormal blood vessel development and lethality in embryos lacking a single VEGF allele[J].Nature,1996,380(6573):435-439.
  • 9Rowe AJ,Wulff C,Fraser HM.Localization of mRNA for vascular endothelial growth factor(VEGF),angiopoietins and their receptors during the peri-Implantaion period and early pregnancy in marmosets(Callithrix jacchus)[J].Reproduction,2003,126(2):227-238.
  • 10Plaisier M,Dennert I,Rost E,et al.Decidual vascularization and the expression of angiogenic growth factors and proteases in first trimester spontaneous abortions[J].Hum Reprod,2009,24(1):185-197.

共引文献1

同被引文献16

引证文献1

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部