南京汉族人群24个Y-STR基因座的遗传多态性

Genetic Polymorphisms of 24 Y-STR Loci in Nanjing Han Population

目的 调查DYS391等24个Y-STR基因座在南京汉族人群的遗传多态性,考察其在法医学中的应用价值。方法 应用AGCU Y-PLUS（24）PCR试剂盒对南京580名汉族无关男性个体进行Y-STR基因座扩增检测分型,用软件计算24个基因座的基因频率等群体遗传学参数,并与湖北、辽宁、广东、北京、成都汉族人群数据进行比较。结果 南京580名汉族无关男性个体在24个Y-STR基因座共发现580种单倍型,各基因座的基因多样性（GD）为0.294 6~0.939 8,单倍型多样性（HD）为0.983 7。六地人群GD值的差异有统计学意义。结论 DYS391等24个Y-STR基因座在南京地区有法医学价值,可用于案件检验及家系排查。

Objective To investigate the genetic polymorphism of DYS391 and other 23 Y-STR loci and to explore its application value in forensic science. Methods Y-STRs loci of 580 unrelated Hart males in Nanjing were amplified using AGCU Y-PLUS PCR （24） kit. The genetic parameters of 24 Y-STR loci such as gene frequency were calculated by software, and compared with the data of Hubei, Liao- ning, Guangdong, Beijing and Chengdu Han population. Results Total 580 haplotypes were detected among 24 Y-STR loci in 580 unrelated Han males in Nanjing. The genetic diversity （GD） of each locus was from 0.2946 to 0.9398, and the haplotypes diversity （HD） was 0.983 7. There was a significant difference between the GD of 6 areas. Conclusion The 24 Y-STR loci such as DYS391 in Nanjing Han population have an application value in forensic science. They can also be used for cases testing and pedigree investigation.