白羊草BiCDPK基因保守区的克隆及序列分析

The Clone and Sequence Analysis of BiCDPK Conserved Sequence from Bothriochloa ischaemum

为了研究CDPK基因的结构和功能,利用RT-PCR方法从白羊草中克隆得到Bi CDPK基因,并对其进行生物信息学分析。结果表明:该Bi CDPK基因c DNA长1048 bp,包含387 bp的开放阅读框,编码128个氨基酸。蛋白表现为弱酸性,且稳定、亲水,二级结构中以无规则卷曲和α-螺旋为主。亚细胞定位于细胞质中,无跨膜结构域,无信号肽。蛋白序列中存在1个丝氨酸磷酸化位点和3个苏氨酸磷酸化位点。进化分析结果表明,克隆的Bi CDPK基因与玉米、小麦和水稻具有较高同源性,相似度分别是97%、86%、86%。为进一步研究Bi CDPK基因的功能奠定了基础。

In order to study the structure and function of CDPK gene, Bi CDPK was cloned from Bothriochloa ischaemum by using RT-PCR method, and analyzed by bioinformatics. The results showedthat the length of c DNA for Bi CDPK was 1048 bp, which contained a 387 bp complete open readingframe encoding 128 amino acids. The corresponding protein was mildly acidic, stable and hydrophilic,with random coil and α- helix as main components in secondary structure. Subcellular localizationindicated that the protein was located in cytoplasmic without transmembrane region and signal peptide.The sequence of protein included one serine phosphorylation sites and three threonine phosphorylationsites. Further phylogenic analysis displayed that Bi CDPK in Zea mays, Triticum aestivum and Oryza sativa shared 97%, 86% and 86% identity. It laid a foundation to further study the Bi CDPK function in Bothriochloa ischaemum.