北极海洋放线菌Streptomyces sp.604F中的抗霉素A和伏尔加霉素类成分

ANTIMYCIN A AND VULGAMYCIN DERIVATIVES FROM THE ARCTIC MARINE ACTINOMYCETE STREPTOMYCES SP.604F

极地微生物代谢产生芳香族硝基化合物、肽类、萜类等多种骨架类型的化学成分。为从北极放线菌中发现结构新颖、活性显著的小分子化合物,本研究采用Sephadex LH-20凝胶和半制备HPLC等柱层析分离纯化化合物手段,结合LC-MS、1H-和13C-NMR等波谱学数据和文献对比进行结构解析,从链霉菌Streptomyces sp.604F提取物中分离鉴定出抗霉素A1b(1)、抗霉素A4a(2)、抗霉素A2a(3)、抗霉素A3a(4)、抗霉素A1a(5)以及伏尔加霉素(6)和8-脱氧伏尔加霉素(7)这7个化合物。化合物1—7是首次从北极海洋沉积物来源的微生物中发现,而且是首次从同一菌株中发现抗霉素A和伏尔加霉素这两类化合物。

Various types of constituents,including aromatic nitro compounds,peptides,and terpenoids,have been reported from different polar microorganisms. In our investigation of secondary metabolites of Arctic actinomycete with new structures or potent bioactivity,five antimycin A derivatives,Antimycin A1b（ 1）,Antimycin A4a（ 2）,Antimycin A2a（ 3）,Antimycin A3a（ 4）,Antimycin A1a（ 5）,together with Vulgamycin（ 6） and its derivative 8-deoxyenterocin（ 7）,were obtained from Streptomyces sp. 604 F. The compounds were purified by Sephadex LH-20 column chromatography and semi-preparative high-performance liquid chromatography,and their structures were determined by comparing their liquid chromatograph-mass spectrometry and1H- and13C-NMR data with those of previously reported compounds. This is the first report of compounds 1-7 from microbes derived from Arctic sediment,and it is the first time that antimycin and vulgamycin compounds were identified as metabolites from the same source.