胃癌组织中幽门螺杆菌感染与增殖、侵袭、血管新生分子的相关性研究

Study on the correlation of helicobacter pylori infection with proliferation,invasion and angiogenesis molecules in gastric cancer tissue

目的:研究胃癌组织中幽门螺杆菌(Hp)感染与增殖、侵袭、血管新生分子的相关性。方法:收集我院就诊的胃癌患者癌组织标本60例以及距离癌组织边缘5 cm以上的正常组织标本60例进行研究,根据Hp检测结果将胃癌组织分为Hp-L(+)和Hp-L(-),测定胃癌细胞增殖相关基因Bcl-2、存活素(Survivin)、激肽释放酶基因8(kallikrein gene 8,KLK8),侵袭相关基因E-cadherin、N-cadherin、Vimentin、Snail、Twist等上皮间质转化基因,血管新生相关基因包括血管内皮生长因子(VEGF)及其受体VEGFR、COX-2、缺氧诱导因子1α(HIF-1α)的含量。结果:胃癌组织中Bcl-2、Survivin、KLK8、N-cadherin、Vimentin、Snail、Twist、VEGF、VEGFR、COX-2、HIF-1α的蛋白含量显著高于正常组织(P<0.05),E-cadherin的蛋白含量显著低于正常组织(P<0.05);Hp-L(+)胃癌组织中Bcl-2、Survivin、KLK8、N-cadherin、Vimentin、Snail、Twist、VEGFR、COX-2、HIF-1α的蛋白含量显著高于Hp-L(-)胃癌组织(P<0.05),E-cadherin的蛋白含量显著低于Hp-L(-)胃癌组织(P<0.05)。结论:胃癌组织中幽门螺杆菌感染能够促进癌细胞的增殖、上皮间质转化以及血管新生。

Objective： To study the correlation of helicobacter pylori infection with proliferation,invasion and angiogenesis molecules in gastric cancer tissue. Methods： A total of 60 cases of cancer tissue samples and 60 cases of normal tissue samples more than 5 cm away from cancer tissue edge were collected for study from gastric cancer patients treated in our hospital,and according to the testing results of helicobacter pylori（ Hp）,gastric cancer tissue was divided into Hp-L（ ＋） and Hp-L（-）,and the levels of proliferation,invasion and angiogenesis molecules were determined. Results： Bcl-2,Survivin,KLK8,N-cadherin,Vimentin,Snail,Twist,VEGFR,COX-2 and HIF-1α protein levels in gastric cancer tissue were significantly higher than those in normal tissue,and Ecadherin protein level was significantly lower than that in normal tissue; Bcl-2,Survivin,KLK8,N-cadherin,Vimentin,Snail,Twist,VEGF,VEGFR,COX-2 and HIF-1α protein levels in Hp-L（ ＋） gastric cancer tissue were significantly higher than those in Hp-L（-） gastric cancer tissue,and E-cadherin protein level was significantly lower than that in Hp-L（-） gastric cancer tissue. Conclusions： Helicobacter pylori infection in gastric cancer tissue can promote cancer cell proliferation,epithelial-mesenchymal transition and angiogenesis.