miR-370在肝癌组织中的表达及与临床病理和预后的关系

miR-370 expression in hepatocellular carcinoma tissue and its relationship with clinical pathology and prognosis

目的：研究miR-370在肝癌组织中的表达及与临床病理和预后的关系。方法：收集肝癌组织和正常组织,测定miR-370、FOXO3a的表达量并分析其与临床病理特征的相关性;培养肝癌细胞,转染miR-370模拟物、FOXO3a过表达质粒后测定细胞的增殖、迁移。结果：肝癌组织中miR-370的表达量显著高于正常组织,FOXO3a的表达量显著低于正常组织且miR-370的表达量与FOXO3a的表达量呈负相关;TNMⅢ~Ⅳ期、有血管侵犯肝癌组织中miR-370的表达量显著高于TNMⅠ~Ⅱ期、无血管侵犯的肝癌组织,FOXO3a的表达量显著低于TNMⅠ~Ⅱ期、无血管侵犯肝癌组织;转染miR-370的模拟物能够促进肝癌细胞的增殖、迁移并降低FOXO3a的mRNA含量;转染miR-370模拟物及FOXO3a过表达质粒后,miR-370模拟物促进肝癌细胞增殖、迁移的效应被削弱。结论：miR-370在肝癌组织中的表达量显著升高且与肿瘤的TNM分期、血管侵犯密切相关,miR-370能够通过靶向抑制FOXO3a的表达来抑制肝癌细胞的增殖和迁移。

Objective： To study the miR-370 expression in hepatocellular carcinoma tissue and its relationship with clinical pathology and prognosis. Methods： Hepatocellular carcinoma tissue and normal tissue were collected to determine miR-370 and FOXO3 a expression levels and analyze their correlation with clinical pathological characteristics; hepatocellular carcinoma cells were cultured and transfected with miR-370 mimics and FOXO3a-overexpression plasmids,and then cell proliferation and migration were detected. Results： miR-370 expression level in hepatocellular carcinoma tissue was significantly higher than that in normal tissue,FOXO3 a expression level was significantly lower than that in normal tissue and miR-370 expression level was negatively correlated with FOXO3 a expression level（ P〈0. 05）; miR-370 expression levels in hepatocellular carcinoma tissue with TNM Ⅲ ~ Ⅳ stage and vascular invasion were significantly higher than those in hepatocellular carcinoma tissue with TNM Ⅰ ~ Ⅱ stage and without vascular invasion,and FOXO3 a expression levels were significantly lower than those in hepatocellular carcinoma tissue with TNM Ⅰ ~ Ⅱ stage and without vascular invasion（ P〈0. 05）; transfection of miR-370 mimics could promote hepatocellular carcinoma cell proliferation and migration and reduce FOXO3 a mRNA content; after transfection of miR-370 mimics and FOXO3a-overexpression plasmids,the effect of miR-370 mimics on promoting hepatocellular carcinoma cell proliferation and migration was weakened. Conclusions： miR-370 expression in hepatocellular carcinoma tissue significantly increases and is closely related to the TNM stage and vascular invasion of the tumor,and miR-370 can targeted inhibit FOXO3 a expression so as to inhibit hepatocellular carcinoma cell proliferation and migration.