人参皂苷Rh2联合吡柔比星调节膀胱癌BIU-87细胞凋亡、侵袭的实验研究

Experimental study of regulation of ginsenoside Rh2 combined with pirarubicin on bladder cancer BIU-87 cell apoptosis and invasion

目的:研究人参皂苷Rh2联合吡柔比星对膀胱癌BIU-87细胞凋亡、侵袭的影响。方法:培养膀胱癌BIU-87细胞并分为对照组、吡柔比星组、人参皂苷组、联合处理组,测定细胞生长、侵袭情况及相关基因的表达量。结果:处理后24、48、72 h时,吡柔比星组、人参皂苷组、联合处理组的细胞生长、侵袭均受到显著抑制,且联合处理组的细胞生长抑制率显著高于吡柔比星组、人参皂苷组(P<0.05),侵袭细胞数目显著少于吡柔比星组、人参皂苷组(P<0.05)。处理后24、48、72 h时,吡柔比星组、人参皂苷组、联合处理组细胞中Bcl-2、CXCR-4的mRNA含量以及上清液中SDF-1的含量显著低于对照组(P<0.05),细胞中Bax的mRNA含量显著高于对照组(P<0.05);联合处理组细胞中Bcl-2、CXCR-4的mRNA含量以及上清液中SDF-1的含量显著低于吡柔比星组和人参皂苷组(P<0.05),细胞中Bax的mRNA含量显著高于吡柔比星组和人参皂苷组(P<0.05)。结论:人参皂苷Rh2联合吡柔比星能够促进膀胱癌细胞的凋亡、抑制膀胱癌细胞的侵袭,Bcl-2/Bax、SDF-1/CXCR-4是药物作用的分子靶点。

Objective： To study the effect of ginsenoside Rh2 combined with pirarubicin on bladder cancer BIU-87 cell apoptosis and invasion. Methods： Bladder cancer BIU-87 cells were cultured and divided into control group,pirarubicin group,ginsenoside group and combined treatment group,and the cell growth and invasion as well as the expression levels of related genes was determined. Results： After treatment for 24 h,48 h and 72 h,cell growth and invasion of pirarubicin group,ginsenoside group and combined treatment group were significantly inhibited and cell growth inhibition rate of combined treatment group was significantly higher than that of pirarubicin group and ginsenoside group（ P〈0. 05）; the number of invasive cells was significantly less than those of pirarubicin group and ginsenoside group（ P〈0. 05）. After treatment for 24 h,48 h and 72 h,the mRNA levels of Bcl-2 and CXCR-4 in cells and SDF-1 levels in supernatant of pirarubicin group,ginsenoside group and combined treatment group were significantly lower than those of control group（ P〈0. 05） and mRNA levels of Bax in cells were significantly higher than that of control group（ P〈0.05）; the mRNA levels of Bcl-2 and CXCR-4 in cells and SDF-1 level in supernatant of combined treatment group were significantly lower than those of pirarubicin group and ginsenoside group（ P〈0. 05） and mRNA level of Bax in cells was significantly higher than those of pirarubicin group and ginsenoside group（ P〈0. 05）. Conclusions： Ginsenoside Rh2 combined with pirarubicin can promote bladder cancer cell apoptosis and inhibit bladder cancer cell invasion,and Bcl-2 / Bax and SDF-1 / CXCR-4 are the molecular targets of drug action.