前列腺癌病理特征与肿瘤组织中MACC1、c-Met、Apaf-1和Caspase-9表达的关系

Relationship between pathological characteristics of prostate cancer and MACC1,c-Met,Apaf-1 as well as Caspase-9 expression in tumor tissue

目的：研究前列腺癌组织中前列腺癌转移相关基因1（MACC1）、c-Met、凋亡蛋白酶活化因子-1（Apaf-1）和细胞凋亡蛋白酶-9（Caspase-9）的表达及其与肿瘤病理特征的关系。方法：选择2015年5月~2016年3月在我院接受手术治疗的前列腺癌和良性前列腺增生患者作为研究对象,收集术中切除的前列腺癌组织和良性前列腺增生组织并测定MACC1、c-Met、Apaf-1、Caspase-9的表达量,收集血清标本并测定miR-let7i、-32、-128、-196a、-218的表达量。结果：前列腺癌组织中MACC1、c-Met的mRNA含量显著高于良性前列腺增生组织（P〈0.05）,Apaf-1和Caspase-9的mRNA含量显著低于良性前列腺增生组织（P〈0.05）,且Gleason分级越高、Whitmore-Prout分期越高,前列腺癌组织中MACC1、c-Met的mRNA含量越高（P〈0.05）,Apaf-1和Caspase-9的mRNA含量越低（P〈0.05）;前列腺癌患者血清中miR-32、miR-128、miR-196a的表达量显著高于良性前列腺增生患者（P〈0.05）,且与Apaf-1、Caspase-9的mRNA含量呈负相关,miR-let7i、miR-218的表达量显著低于良性前列腺增生患者（P〈0.05）,且与MACC1、c-Met呈负相关。结论：MACC1、c-Met高表达、Apaf-1和Caspase-9的低表达与前列腺癌的发生以及病情发展相关,前列腺癌组织中MACC1、c-Met、Apaf-1、Caspase-9的表达受到miRNAs的调控。

Objective： To study the MACC1,c-Met,Apaf-1 and Caspase-9 expression in prostate cancer tissue and their relationship with the pathological characteristics of tumor. Methods： Prostate cancer and benign prostatic hyperplasia patients who received surgical treatment in our hospital from May 2015 to March 2016 were selected as the research subjects,prostate cancer tissue and benign prostatic hyperplasia tissue were collected during surgery to determine MACC1,c-Met,Apaf-1 and Caspase-9 expression,and serum specimens were collected to determine miR-let7 i,-32,-128,-196 a and-218 expression levels. Results： mRNA content of MACC1 and c-Met in prostate cancer tissue were significantly higher than those in benign prostatic hyperplasia tissue while mRNA content of Apaf-1 and Caspase-9 were significantly lower than those in benign prostatic hyperplasia tissue,and the higher the Gleason grading and the higher the Whitmore-Prout staging,the higher the mRNA content of MACC1 and c-Met in prostate cancer tissue and the lower the mRNA content of Apaf-1 and Caspase-9; serum miR-32,miR-128 and miR-196 a expression levels in prostate cancer patients were significantly higher than those in patients with benign prostatic hyperplasia and negatively correlated with the mRNA content of Apaf-1 and Caspase-9,and the expression levels of miR-let7 i and miR-218 were significantly lower than those in patients with benign prostatic hyperplasia and negatively correlated with MACC1 and c-Met. Conclusions： High MACC1 and c-Met expression and low Caspase-9 and Apaf-1 expression are related to the occurrence and progression of prostate cancer,and the MACC1,c-Met,Apaf-1 and Caspase-9 expression in prostate cancer tissue are regulated by miRNAs.