摘要
为了建立一种高效液相色谱测定环丙酰草胺含量的分析方法,为农药登记提供科学的评估数据。采用高效液相色谱法,使用ZORBAX SB-C18色谱柱,以甲醇＋水（含2%冰乙酸）=90＋10（V/V）为流动相,流速为1m L/min,在255nm波长下对环丙酰草胺进行定量分析。结果表明当环丙酰草胺质量浓度在25-400 mg/L范围内,高效利用液相色谱法的线性相关系数为0.999 1,精密度试验相对标准偏差为0.20%,平均回收率为：99.2%。该分析方法准确、快速,适用于环丙酰草胺定量分析。
An HPLC method was developed for the quantitative determination of cyclanilide. The result would providescientific data for pesticide registration. The content of active ingredients in cyelanilide was determined quantitatively byHPLC with ZORBAX SB C18 column at UV 255 nm,and methanol-water（90 ： 10,V / V） as mobile phase at a flow rateof 1 mL / min. The assay exhibited a good linearity when the eyclanilide concentration was from 25 to 400 mg / L with acoefficient of 0. 9991. The relative standard deviation was 0. 20 % and the average recovery was 99.2 %. The method issimple and accurate, and is suitable for the quantitative analysis of cyelanilide.
出处
《贵州科学》
2016年第4期61-64,共4页
Guizhou Science
