急性胰腺炎胰腺腺泡细胞转分化的初步研究

A preliminary study on trans-differentiation of pancreatic acinar cell in acute pancreatitis

目的:检测跨膜蛋白DC-SIGN在急性胰腺炎中的表达,探讨急性胰腺炎胰腺腺泡细胞的转分化现象。方法:采用AR42J细胞株建立急性胰腺炎的体外细胞模型。流式细胞法检测细胞表面DC-SIGN蛋白的表达量,RTPCR检测DC-SIGN m RNA转录水平。结果 :在蛋白质和基因水平,腺泡细胞DC-SIGN在急性胰腺炎早期高表达(P<0.05)。结论:急性胰腺炎胰腺腺泡细胞DC-SIGN高表达,表明存在上皮-树突状细胞转分化现象。

Objective To investigate the trans-differentiation of pancreatic acinar cell in acute pancreatitis（AP） by detection of the expression of transmembrane protein that is dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin（DC-SIGN）. Methods An in vitro study was conducted using AR42 J cell line for establishing the cell model of AP. The expression level of DC-SIGN protein was measured by flow cytometry. The transcription level of DCSIGN m RNA was detected by RT-PCR. Results DC-SIGN in early phase of AP was characterized by high expression in gene and protein level（ P〈 0. 05）. Conclusions The high expression of DC-SIGN on pancreatic acinar cells during AP demonstrates the epithelial-dendritic cell trans-differentiation.