聚焦超声治疗小鼠CINⅠ的疗效

Effects of focused ultrasound on grade I cervical intraepithelial neoplasia in mice

目的 :探讨聚焦超声治疗小鼠宫颈上皮内瘤样变(cervical intraepithelial neoplasia,CIN)的疗效及其可能的作用机制。方法 :利用二甲基苯蒽原位诱发小鼠CINⅠ动物模型,随机分为治疗组(20只)和对照组(20只)。治疗组给予聚焦超声治疗;对照组采用与治疗组相同的操作方法进行治疗,但仪器无功率输出。治疗后2周,应用HE染色法和免疫组织化学法分别检测小鼠宫颈组织的病理学变化以及小鼠宫颈组织中p16、Ki67、Fas和Fas L的表达。结果 :成功建立了小鼠CINⅠ动物模型。治疗组中有15只小鼠治疗后恢复为正常宫颈组织,4只小鼠治疗后仍为CINⅠ,死亡1只,治疗有效率为75%(15/20);对照组中有11只小鼠治疗后仍为CINⅠ,6只发展为CINⅡ,1只发展为CINⅢ,死亡2只,治疗有效率为0%;2组间差异有统计学意义(P<0.01)。治疗组小鼠宫颈组织中p16、Ki67和Fas L的强阳性表达率低于对照组,而Fas的强阳性表达率高于对照组(P值均<0.01)。结论 :聚焦超声可有效治疗小鼠CINⅠ,这可能与宫颈组织中p16、Ki67、Fas和Fas L表达水平的变化有关。

Objective： To investigate the effect of focused ultrasound on murine cervical intraepithelial neoplasia （CIN） and its possible mechanism. Methods： The mice bearing grade I CIN were orthotopically induced by dimethl benz anthracene and randomly divided into treatment group （n = 20） and control group （n = 20）. The mice in the treatment group were treated with focused ultrasound, while the mice in the control group were treated with the same method but without power output. After two weeks, the pathological changes and the expressions of p16, Ki67, FasL and Fas in murine cervical tissues were detected by HE staining and immunohistochemistry, respectively.Results： The model of grade I CIN in mice was successfully established. In the treatment group, the cervical tissues returned to normal in 15 mice after treatment, 4 mice remained grade I CIN, and 1 mouse died. The response rate of treatment group was 75% （15/20）. In the control group, the cervical tissues remained grade I CIN in 11 mice after treatment, 6 mice developed to grade II CIN, 1 mouse developed to gradelII CIN, and 2 mice died. The response rate of the control group was 0% （0/20）. There was a significant difference between the treatment group and the control group （P 〈 0.01）. The strong positive expression rates of p16, Ki67 and FasL in cervical tissues in the treatment group were lower than those in the control group, while the strong positive expression rate of Fas was opposite （all P 〈 0.01）. Conclusion： The focused ultrasound can effectively treat the grade I CIN in mice. This effect may be related to the changes of p16, Ki67, Fas and FasL expressions.