钛表面接枝溴代十六烷后的杀菌效果研究

Effect of grafting 1-Bromohexadecane onto titanium surface on bactericidal ability

目的在钛表面沉积透明质酸(HA)和壳聚糖(CHI)聚电解质多层膜,并用溴代十六烷对CHI上的氨基季铵化,以评价其杀菌效果。方法在碱化处理过的钛片上吸附带正电荷的聚乙烯亚氨(PEI),再用层层自组装的方法在钛表面交替沉积带负电荷的HA和带正电荷的CHI,并用溴代十六烷(C16H33Br)对CHI上的氨基季铵化,形成Ti-PEI-(HA-CHI)20-N+(C16H33)3Br-涂层,扫描电子显微镜(SEM)对涂层断面进行表征;以纯钛为对照组,Ti-PEI-(HA-CHI)20和Ti-PEI-(HA-CHI)20-N+(C16H33)3Br-为实验组,分别在其表面进行变形链球菌(S.m)培养24h后用荧光显微镜和SEM检测钛片表面的细菌活性。结果SEM显示聚电解质多层膜已沉积到钛片表面并具有一定的厚度。荧光显微镜显示纯钛表面有大量的活细菌,Ti-PEI-(HA-CHI)20上细菌数量较少,且有部分死菌;而Ti-PEI-(HA-CHI)20-N+(C16H33)3Br-上细菌几乎全为死菌。SEM结果显示纯钛、Ti-PEI-(HA-CHI)20、Ti-PEI-(HA-CHI)20-N+(C16H33)3Br-3组钛片上的细菌数量依次减少。结论钛表面沉积HA/CHI聚电解质多层膜,并对多层膜中的CHI季铵化后,其表面具有显著的杀菌作用。

Objective To evaluate the effect of grafting 1-Bromohexadecane onto polyelectrolyte multilayer films assembled by HA and CHI of Titanium （Ti） surface on bactericidal ability. Methods Firstly, the poly（ethylene imine）（PEI） was deposited on Ti surface, and then polyelectrolyte multilayer films were prepared by layer-by-layer assembly with HA and CHI, subsequently the Ti-PEI-（HA-CHI）20-N＋（ Cr6H33）3Br coatings formed by the quaternization CHI with 1-Bromohexadecane. The Ti sample embedded in resin was observed under SEM. After the culture of Streptococcus mutans （S.m） on the Ti surface for 24 hours, the bactericidal ability was tested by fluorescence microscopy and SEM. Results SEM showed the PEI-（HA-CHI）20- C16H33Br was successfully deposited on Ti surface. Fluorescence microscopy showed that a large number of live bacterial on bare Ti, and on Ti-PEI-（HA-CHI）20 surface, the number of bacteria was less than that on the bare Ti, and some of the bacteria were dead ; while on the Ti-PEI-（HA-CHI）20-N＋（ CIaH33）3Br-surface, the number of bacteria was dramatically decreased and the dead,bacteria were in large proportion. The SEM results showed that the number of the bacteria decreased in the order of bare Ti, Ti-PEI-（HA-CHI）20 and Ti-PEI-（HA-CHI）20-N＋（ CI6H33）3Br-. Conclusions The HA/CHI polyelectrolyte multilayer films were deposited on titanium surface. After forming Ti-PEI-（HA-CHI）20-N＋（ C16H33）3Br coatings by quaternization CHI with 1-Bromohexadecane, the surface had significant bactericidal ability.