高效液相法测定头孢拉定胶囊流动相条件的改进

Improvement of mobile phase conditions of cephradine capsules by HPLC

寻找一种快速的,对高效液相色谱仪和色谱柱损害最小的流动相条件,使其适用于头孢拉定胶囊含量测定的学生实验。方法:通过改变流动相中水,甲醇,3.86%醋酸钠溶液,4%醋酸溶液的比例,寻找最优的流动相条件。结果:最优流动相条件为水-甲醇-3.86%醋酸钠溶液-4%醋酸溶液(1480∶640∶2∶0.4)。运用此流动相测定头孢拉定胶囊,方法学符合标准,与药典方法相比,用时短,对液相和色谱柱损害小。结论:此流动相适合头孢拉定胶囊的含量测定,可以用于学生实验。

Objective： To search a mobile phase condition which fast and minimal damage to the high performance liquid chromatography and the column,making it suitable for student experiments of the content determination of cephradine capsules. Methods： By changing the mobile phase ratio of water,4% acetic acid solution,3. 86% sodium acetate solution and methanol,to find the optimal mobile phase conditions. Results： The optimal condition for the mobile phase water- methanol-3. 86% sodium acetate-4% acetic acid（ 1480 ∶ 640 ∶ 2 ∶ 0. 4）. The mobile phase was used for measuring cephradine capsules,the methodology meet the standard,to compare with the pharmacopoeia method,saving much time and little damage to the instrumentation and column. Conclusion： This mobile phase is applicable to the determination of cephradine capsules,which can be used for student experiments.