改性淫羊藿苷共价结合壳聚糖/聚羟基丁酸酯-羟基戊酸酯构建组织诱导型骨修复支架材料

Modified Icariin covalent with chitosan/poly hydroxybutyrate-co-hydroxyvalerate to fabricate tissue inducible bone repair scaffold material

为构建一种诱导型骨修复支架材料,将淫羊藿苷（Ica）经过氨基化改性得到Ica-NH2,并对Ica-NH2进行FTIR、XRD和TG-DTA表征。以Ica-NH2共价结合壳聚糖（CS）为诱导因子添加方式,聚羟基丁酸酯-羟基戊酸酯（PHBV）和CS为基材,经过两相混合急速冷冻/冷冻干燥成型技术制备了Ica-NH2-CS/PHBV骨组织工程支架。随后,对支架材料进行了SEM、体外药物缓释、力学性能、细胞相容性及细胞增殖评价。结果显示：氨基化改性Ica的FTIR谱图在3 371、3 328cm-1处出现2个中等强度、尖锐的N—H伸缩振动峰,在1 689cm-1处出现N—H变角振动峰;XRD谱图显示其衍射增强且整体左移,证明Ica被成功氨基化改性;支架材料显微结构呈网络状串珠状,并均匀分布着3~10μm的微孔,其体外药物缓释效果良好,力学强度介于硬质骨与松质骨之间,复合细胞培养7d后表现出良好的贴附与增殖,且细胞在Ica-NH2-CS/PHBV支架材料上的增殖增长率显著高于CS/PHBV。研究表明所构建的Ica-NH2-CS/PHBV支架材料可作为一种良好的诱导型骨修复材料。

In order to fabricate an inducible bone repair scaffold materials,Icariin（Ica）were modified by amino and then got Ica-NH2,Ica-NH2 was characterized by FTIR,XRD and TGA.Ica-NH2 covalent binding with carboxymethyl chitosan（CS）as the inducing factor addition method,poly hydroxybutyrate-co-hydroxyvalerate（PHBV）and CS were used as basic materials,then two-phase mixed rapid freezing/freeze-drying technology was used to build the Ica-NH2-CS/PHBV bone tissue engineering scaffolds.And then the scaffold materials were evaluated by SEM,invitro drug release,mechanical properties,cell compatibility and proliferation.The results show that：there were two moderate intensity,sharp N—H stretching vibration peaks at 3 371 and 3 328cm-1 in the FTIR spectra of aminated Ica,one N—H waving vibration peaks appears at 1 689cm-1,XRD pattern shows the diffractions are enhanced and move to the left as a whole,these results indicate that Ica is successfully amino-modified.The scaffold materials possess network-and bead-like mircostructures,which are uniformly distrubuted wtih microporous of3-10μm.Ica-NH2-CS/PHBV scaffold has good invitro drug release effect.Mechanical strength tests show its mechanical strength is between dense bone and cancellous bone,composite cells closely adhere on the scaffold after 7d cultivation and proliferate well,and the proliferation rate of cells attached on Ica-NH2-CS/PHBV scaffold materials is significantly higher than that on the CS/PHBV.This research indicates that Ica-NH2-CS/PHBV scaffold materials could be used as good type of inducible bone repair materials.