血管内皮钙黏蛋白的表达与CASP脓毒症大鼠肠系膜微循环的关系

Association between the expression of ve-cadherin and mesentery mi- crocirculation blood flow of sepsis CASP rats and its clinical significance

目的通过监测血管内皮钙黏蛋白的表达并测定CASP脓毒症大鼠肠系膜微循环的血流速度，分析相互之间的关系。方法参照脓毒症CASP脓毒症大鼠造模标准造模，根据静脉留置针孔径分组，每组6只，脓毒症A组22G（0．9mm×25mm，33ml／min），脓毒症B组20G（1．1mm×32mm，54mE／min），脓毒症C组18G（1．3mm×32mm，80ml／min），脓毒症D组14G（2．0mm×45mm，270ml／min），另外设置对照组（无操作组，n=6），运用活体显微镜技术测定各组脓毒症大鼠6h的血流速度，免疫组化半定量分析各组大鼠肠系膜钙黏蛋白的表达。结果对照组肠系膜微循环血流速度为（583．21±52．39）μm／s，较脓毒症D组（213．30±52．39）μm／s明显升高（P〈0．05），脓毒症A组（482．71±58．62）μm／s，较脓毒症D组明显升高（P〈0．05）。对照组肠系膜钙黏蛋白免疫组化半定量评分（11．17±0．34）分，较脓毒症D组（5．43±0．43）分明显升高（P〈0．01），脓毒症A组（10．07±0．30）分，较脓毒症D组明显升高（P〈0．05）。结论CASP脓毒症大鼠肠系膜钙黏蛋白的表达量与膜微循环血流速度呈正相关，可间接反映脓毒症病变程度。

Objective To monitor the association between the expression of ve-cadherin and mesentery microcircula- tion blood flow of sepsis CASP rats, Analysis the relationship of each other. Methods Based on the standard criteria,we established sepsis CASP rats, and the rats were divided into several groups according to the gauge of the vein detained needle in the ascending colon,every group has six rats. The groups consisted of Sepsis A group 22 G（0.9 mm× 25 mam, 33 ml/nin）, sepis B group 20 G（1.1 mm ×32 mm, 54 ml/min）, sepsis C group 18 G（1.3 mm ×32 mm, 80 ml/min）, sepsis D group 14 G（2.0 mm ×45 mm, 270 ml/min）, in addition we established the control group. Real-time observa- tion was performed by microscope on the 6 h＇s blood velcocity of each sepsis CASP rats group. Then we applied the im- munohistochemistry to quantitatively analyze the expression of ve-cadherin of each group. Results The blood velcocity of the mesentery microcirculaticn in control group was （583.21 ±52.39） μm/s, it was higher compared with the sepsis D group （213.30 ± 52.39） μm/s （ P 〈 0.05 ）, the blood velocity in sepsis A group was （482.71 ± 58.62 ） μm/s, higher compared with the sepsis D group（P 〈0.05）. The score of the immunohistochenfistry quantitative analysis of mesentery ve- cadherin in control group was 11.17 ± 0.34, higher compared with the sepsis D group （5.43 ± 0.43） （ P 〈 0 .01 ）. The score of sepsis A group was 10.07 ± 0.30, higher compared with the sepsis D group （ P 〈 0.05 ）. Conclusions The ex- pression of re- cadherin of sepsis CASP rats had positive correlation with the blood velocity of the mesentery microcireula- tion of sepsis CASP rats,and it can indirectly reflect the degree of the sepsis.