梅毒螺旋体酶联免疫吸附试验和甲苯胺红不加热血清试验在梅毒检测中的应用

Compare analysis of TP-ELISA and TRUST in syphilis testing

目的探讨梅毒螺旋体酶联免疫吸附试验(TP-ELISA)和甲苯胺红不加热血清试验(TRUST)在梅毒检测中的应用特点。方法对2015年1月至12月22 328例门诊及住院患者血清标本同时进行ELISA和TRUST试验检测梅毒螺旋体抗体,并比对分析检测结果。结果 22 328份患者血清标本中,经ELISA和TRUST检测,共检出阳性950例,ELISA阳性912例,TRUST阳性370例,两种方法均为阳性者332例。两种方法比较,差异有统计学意义(P<0.001)。结论 ELISA可用作为梅毒的筛查试验,TRUST滴度分析可作为疗效观察,判定复发或再感染指征。两方法结合检测对加强梅毒监测、诊断和预后均有一定的意义。

Objective To investigate the effects of treponema pallidum adsorption test （ELISA） and toluidine red unheated serum test （TRUST） in syphilis testing. Methods Tested 22328 patients serum specimens with TP-ELISA and TRUST, made a compare analysis of the test results. Results In 22328 patients, total positive results were 950 cases,912 cases were ELISA positive,370 cases were TRUST positive and 332 cases were both positive.Compare analyzed the two methods,the difference was statistically significant（P〈0.O01）. Conclusion TP-ELISA is available for syphilis screening test,and TRUST can be used as a indication for clinical observation,relapse or reinfection judgment, Two methods in combination with syphilis detection has a certain significance,especially in monitoring strengthening,iudgment and prognosis of syphilis.