LC-MS/MS法测定大鼠血浆中的罗哌卡因

Determination of ropivacaine in rat plasma with liquid chromatography-tandem mass spectrometry

目的建立测定大鼠血浆中罗哌卡因的高效液相色谱串联质谱法。方法色谱柱为Agela MP C18（2.1 mm×50 mm,3μm）。采用电喷雾离子源（ESI）正离子多反应监测（MRM）扫描分析,罗哌卡因和内标（咪达唑仑）的检测离子分别为m/z 275.2/126.1和326.0/291.0。结果罗哌卡因的线性范围为0.5~250 ng/ml,相关系数r为0.9946,批内精密度为1.32%~6.74%,基质效应在95.5%~102.6%,回收率为89.8%~104.7%。结论本法操作简单、方法准确可靠、检测灵敏度高,可用于罗哌卡因大鼠体内的药代动力学研究。

Objective To develop an LC-MS/MS method for the determination of ropivacaine in rat plasma. Methods The chromatographic separation was achieved on an Agela MP C18（2.1 mm×50 mm,3 μm）analytical column. The multiple reaction monitoring（MRM）mode of the positive ion was adopted in the MS detection,and the precursors to the product ion transitions of m/z 275.2/126.1and 326.0/291 were used to measure ropivacaine and internal standard（midazolam）. Results The method was linear over the ropivacaine concentration range of 0.5-250 ng/ml with the correlation coefficients r of 0.9946. The average inter-day precision values（RSD）were 1.32%-6.74%,the matrix effect values were 95.5%-102.6% and the average recovery values were 89.8%-104.7%. Conclusion The analysis methods for ropivacaine are simple,accurate and sensitive,thus can be used for the pharmacokinetic research of ropivacaine incorporated cubic liquid crystal in rat.