甲苯咪唑在鲫体内的药动学及残留消除研究

Pharmacokinetics and Residue Elimination of Mebendazole in Crucian Carp Carassius auratus

在水温(20±0.5)℃下,给体质量(50±10)g的鲫Carassius auratus单剂量口灌20mg/kg(体质量)甲苯咪唑后0.25h、0.5h、0.75h、1h、1.5h、2h、4h、6h、8h、12h、24h、36h、48h,及72h,采集鲫血浆,利用高效液相色谱法测定血浆中药物的浓度,药动学DAS3.0软件进行数据分析和处理,研究甲苯咪唑在鱼体内的吸收和消除规律。结果表明:单次口灌给药后,甲苯咪唑在血浆中的药时关系符合一级吸收二室开放模型。每天给药一次,连续3d,氨基甲苯咪唑(MBZ-NH2)于25d后低于检测限(0.0465μg/m L)。在本试验条件下,建议休药期不低于25d。

The pharmacokinetics and residue elimination of mebendazole（MBZ）were studied in crucian carp Carassius auratus with body weight of（50 ± 10）g exposed to oral administration of MBZ at dose of 20 mg/kg bodyweight by a means of high performance liquid chromatography（HPLC）at water temperature of（20 ± 0.5）℃. The drug concentrations in plasma were monitored in the crucian carP〈0.25 h, 0.5 h, 0.75 h, 1 h, 1.5 h, 2 h, 4 h, 6 h, 8 h, 12 h, 24 h, 36 h, 48 h, and 72 h after drug administration, and analyzed by DAS 3.0 software. The results showed that the concentrations versus time of mebendazole in muscle were well described by two-department open model with first-order absorption after a single oral administration. The residues in the crucian carp by oral administration once a day for 3 d were found to be less than the maximum residue limit（MRL）（0.0465 μg/m L）at the 25 d for MBZ. The findings indicate that the withdrawal time should be no less than 25 d after administration under these conditions.