白藜芦醇对血管紧张素Ⅱ诱导心肌肥大的抑制作用

Resveratrol inhibits angiotensin Ⅱ- induced cardiac hypertrophy

目的:研究白藜芦醇(Resveratrol Resv)对血管紧张素II(Angiotensin II,Ang II)诱导H9C2细胞肥大的抑制作用,并探讨其机制。方法:通过建立Ang II诱导H9C2细胞肥大模型,采用Image Pro Plus 6.1软件测量细胞表面积大小;BCA法测定细胞总蛋白含量,Western-blot方法检测H9C2细胞p-CREB和CREB蛋白的表达。结果:与对照组相比,Ang II(0.1μM)孵育后,细胞表面积增加,细胞蛋白含量增加了46%(P<0.01)。Resv(0.01μM+Ang II组,0.1μM+Ang II组,1μM+Ang II组)能浓度依赖地减少蛋白含量。Ang II诱导H9C2细胞p-CREB蛋白的表达升高。不同剂量Resv对Ang II诱导H9C2细胞p-CREB蛋白的表达均有抑制作用,并且呈浓度依赖。结论:Resv抑制Ang II介导的H9C2细胞肥厚,其机制可能是通过抑制细胞表面积增大、细胞蛋白含量增多和CREB蛋白表达实现的。

Objective： To investigate the inhibitory effects of resveratrol on Ang II -induced cardiac hy- pertrophy in H9C2 cells, so as to elucidate the role of ERK1/2 - CREB signaling pathway during cardiac hy- pertrophy. Methods： Ang II was applied to establish the cardiacmyocytes hypertrophy model of H9C2 cells. The Cell surface area of H9C2 cells was measured by Image Pro Plus 6. 1 software. The total protein content of H9C2 cells was detected by BCA method. The protein expression of CREB and p - CREB were determined by western blot. Results： Compared with the control group, cell surface area was increased significantly in Ang II group （P 〈 0. 01 ）. The total protein content of the cells was also increased significantly in Ang II group （P 〈 0. 01 ）. The total protein content of Resv group （0. 01μM, 0. 1 μM, 1 p.M ＋ Ang II ） was decreased in a concentration - dependent manner. Ang II stimulated phosphorylation of CREB. Resv （0. 01μM, 0. 1 μM, 1μM ＋ Ang II group） could reduce the expression level of p - CREB in a concentration - dependent manner. Conclusion： Resv could inhibit AngII induced cardiac hypertrophy, and its mechanism might be through inhibiting the increases of protein content, surface area and the expression of CREB.