Ki67与核酸荧光共染在细胞周期研究中的应用

Application of Ki67 and nuclear acid fluorescent dye co-staining in analysis of cell cycle

病原微生物与宿主细胞的相互作用是感染过程中的一个重要环节,也是研究肿瘤生物学的一项重要内容。动态研究细胞周期变化对了解病原体作用于细胞具有重要意义。本研究用Ki67/4′,6-二脒基-2-苯基吲哚(4′,6-diamidino-2-phenylindole,DAPI)和Ki67/碘化丙啶(propidium iodide,PI)共染技术分析了小鼠脾细胞的细胞周期变化,并介绍其具体应用。

The interaction between pathogenic microbes and host cells is crucial for studies on infections and tumor cell biology. The kinetic studies of cell cycle are of significance for understanding the mechanisms mediated by microbes versus host cells. In this paper, a novel method for cell cycle analysis in mouse splenic cells by Ki67 and 4＇ ,6-diamidino-2-phenylindole （DAPI） or propidium iodide （PI） co-staining is introduced.