三维血流流场成像与斑块破裂的力学机制2011年度报告

Three Dimensional Hemodynamic Imaging and Mechanisms of Atherosclerotic Plaque Rupture

该年度预期研究目标包括构建si RNA,培养人脐静脉内皮细胞,完成各分组,给予不同的剪切力观察不同的剪切力观察信号转导通路膜整合素→MAPKs→核转录因子→MMP和P4Hα1及底物的关系。同时观察炎性因子的改变;建立动脉粥样硬化小型猪模型,获取斑块的OCT或光声图像,进行图像和回波波谱分析,对比组织学实验结果,建立弹性数据和斑块组织成分间的映射关系;结合真实血管三维重建构型,利用Comsol Multiphysics有限元软件建立仿真模拟系统。建立兔动脉粥样硬化动物模型,定期检测获取不同阶段的相关数据。主要研究发现包括:(1)证实斑块部位不同的机械应力特征与不同的病理学变化具有显著相关性;(2)首次提出了细胞内NADPH氧化酶介导的氧化还原信号传导机制可能是细胞应激反应体系中一个固有的组成部分;(3)尝试利用显微超声粒子图像测速技术(Micro Ultrasonic Particle Image Velocimetry)获得血管内血流场速度矢量分布。

This year we aims to construct siRNA, culture HUVEC cells and test the effects of shear, stress on signaling pathways including integrin-〉MAPKs-〉transcription factors-〉MMP and P4Hc（1 expression, and observe the changes in inflammatory responses; Obtain OCT or optical-sonic images and perform image or echo spectrum analysis, and compare the results with real histology images; clarify the relationships between plaque elasticity data and the biological components in the plaquesl Using Comsol Multiphysics software to create hemodynamic simulation platform, and test the correlations in rabbit models of atherosolerosis. Our major findings include：（1）we have shown that mechanic properties of atherosclerotic plaque ere correlated with histology characteristicsI（2）we propose NADPH oxidase- dependent redox signaling may be a critical component in cellular stress response;（3）we attempted to use Micro Ultrasonic Particle Image