泌乳奶牛尾动脉和阴外动脉血浆中氨基酸含量的比较

Comparison of Three Model of Diets on Amino Acids Content in Plasma of Caudal Artery and External Pudic Artery of Lactating Dairy Cows

目的该试验旨在通过对三种日粮模式下泌乳奶牛尾动脉和阴外动脉血浆中氨基酸含量的比较,探讨在乳腺对氨基酸摄取效率等研究中是否可以用尾动脉血代替阴外动脉血。方法选用体况良好,体重相近(554±21)kg,产奶量24.30±kg/d左右,荷斯坦奶牛30头,分为3组,每组10头。试验设计为完全随机试验设计,饲喂日粮分别为苜蓿+全株玉米青贮+羊草和混合粗饲料日粮组(MF),概略养分水平约同MF秸秆日粮组(CSA)和秸秆全部替代MF组粗饲料日粮组(CSB)。试验持续28d,试验期最后2d分别采集阴外动脉和尾动脉血样,并测定尾动脉及阴外动脉血浆中游离氨基酸的含量。结果结果表明:三种不同日粮模式下尾动脉与阴外动脉血浆中大多数单一氨基酸含量差异均不显著(>0.05),只有少数氨基酸含量差异显著(<0.05)。结论从以上结果得出,在一定情况下可以用尾动脉血代替阴外动脉血研究乳腺对氨基酸的摄取效率。

Objective The experiment was conducted to adopt the comparison of three model of diets on amino acids content in plasma of caudal artery and external pudic artery of lactating dairy cows,discuss whether could replace by blood of caudal artery instead of external pudic artery on amino acid uptake of mammary gland efficiency. Method Thirty Holstein dairy cows at good body condition and similar body weight and milk yield was 25 kg/d into three groups（MF, CSA, CSB）, each group of ten. The experiment designed for completely randomized. Feeding different diets to dairy cows, MF was lucerne and whole crop corn silage and guinea grass and mix with roughage group, CSA was straw that General nutrition level was MF group, CSB was straw roughage instead of MF group. Test lasted 28 d ,blood of caudal artery and external pudic artery was collected in the last 2 d, take measure of content in plasma of FAA Result The results indicated that there was no significant difference in the seport of most single amino acids content in plasma of caudal artery and external pudic artery（P〉0.05）. Just few was significant difference（P〈0.05）. Conclusion It could be seen that it could replace by blood of caudal artery instead of external pudic artery on amino acid uptake of mammary gland efficiency .