血清TGF-β1、HIF-1α、sFlt-1联合检测在子痫前期患者中的临床意义

Clinical significance of combined detection of serum TGF-β1,HIF-1 and sFlt-1 in patients with preeclampsia

目的探讨血清血清转化生长因子β1(TGF-β1)、低氧诱导因子1α(HIF-1α)、可溶性血管内皮生长因子受体1(sFlt-1)联合检测在子痫前期患者中的临床意义。方法选择2014年3月至2015年3月该院接诊的重度子痫前期患者50例、轻度子痫前期患者50例及正常妊娠孕妇50例,比较3组孕妇血清TGF-β1、HIF-1α、sFlt-1水平及胎盘组织HIF-1α相对吸光度比值、sFlt-1mRNA和蛋白水平,同时比较轻度、重度子痫前期患者不良妊娠结局发生情况。结果重度子痫前期患者血清TGF-β1、HIF-1α、sFlt-1水平高于轻度子痫前期患者和正常妊娠孕妇(P<0.05)。重度子痫前期患者HIF-1α相对吸光度比值、sFlt-1mRNA和sFlt-1蛋白水平高于轻度子痫前期患者和正常妊娠孕妇(P<0.05);重度子痫前期患者不良妊娠结局总发生率高于轻度子痫前期患者(P<0.05)。结论 TGF-β1、HIF-1α、sFlt-1参与了子痫前期的发生、发展,3种血清标志物联合检测可有效反映患者病情进展程度,可为判断患者预后提供可靠的依据。

Objective To investigate the clinical significance of serum transforming growth factor-β1（TGF-β1） ,hypoxia induc-ible factor-1α（HIF-1α） and soluble fms-like tyrosine kinase receptor 1（sFlt-1） in patients with preeclampsia .Methods From March 2014 to March 2015 ,50 cases of severe preeclampsia ,50 cases of mild preeclampsia and 50 cases of normal pregnancy were enrolled and detected for serum TGF-β1 ,HIF-1α and sFlt-1 ,relative absorbance ratio of HIF-1α and sFlt-1 mRNA and protein .The adverse pregnancy outcomes of patients with severe and mild preeclampsia were compared .Results Serum TGF-β1 ,HIF-1 and sFlt-1 levels in severe preeclampsia group were higher than mild preeclampsia group and normal pregnancy group（P〈 0 .05） .The relative absor-bance ratio of HIF-1 ,sFlt-1 mRNA and sFlt-1 protein levels in severe preeclampsia group were higher than mild preeclampsia group and normal pregnancy group（P〈 0 .05） .The total incidence of adverse pregnancy outcomes in severe preeclampsia group was higher than preeclampsia group（P〈 0 .05） .Conclusion TGF-β1 ,HIF-1α and sFlt-1 could be with important role in the treatment of pa-tients with preeclampsia .The combined detection of these three serum markers could be used for the prognosis of patients with pre-eclampsia .