佳宝苦瓜杂交种纯度的SRAP鉴定

Hybrid Purity Identification of Jiabao Bitter Gourd by SRAP Markers

利用SRAP技术对佳宝苦瓜杂交种子进行纯度鉴定。试验结果表明,从153对SRAP引物中筛选出1对引物Me6-Em4,能同时扩增出F_1代及其父母本的多态性条带,且PCR扩增产物具有高度的稳定性和重复性;利用该引物对48株佳宝苦瓜进行纯度鉴定,其种子纯度为97.9%,与其田间鉴定结果接近,表明利用SRAP分子标记可以快速、准确地鉴定佳宝苦瓜杂交种纯度。

In Lhis sLudy, SRAP markers Lechnique was used for idenLifying Lhe puriLy of Jiabao biLLer gourd hybrid seeds. The resulLs showed LhaL only a pair of primers （Me6-Em4） was screened ouL from 153 pairs of SRAP primers, which could amplify Lhe characLerisLic polymorphic bands of F｜ generaLion and iLs parenLs, and Lhe PCR producLs possessed high sLabiliLy and repeaLabiliLy. Using Lhe pair of primers Lo deLecL Lhe puriLy of 48 individuals of Jiabao biLLer gourd, Lhe resulLs showed LhaL Lhe puriLy was 97.9% for hybrid seeds of Jiabao biLLer gourd, which was very close Lo Lhe field morphological LesL , indicaLing LhaL SRAP markers could be used Lo idenLify Lhe hybrid puriLy of Jiabao biLLer gourd rapidly and accuraLely.