葛根素干混悬剂的制备及其含量测定研究

Preparation and Content Determination of Puerarin Dry Suspension

目的：研制葛根素干混悬剂,并建立紫外分光光度法对其含量进行测定。方法：以葛根素为主药,Avicel CL611、泊洛沙姆、十二烷基硫酸钠为辅料,通过正交试验设计,优化工艺处方制备葛根素干混悬剂;并采用紫外分光光度法测定该制剂中葛根素的含量。结果：制备处方合理,工艺可靠。制剂中葛根素的含量测定的线性范围为0.00303-0.0101mg/ml,平均加样回收率为99.60%,平均RSD为2.38%。结论：优化后的制备工艺稳定可控,重复性好。含量测定方法简便准确,专属性强,可用于葛根素干混悬剂的质量控制。

Objective To prepare Puerarin dry suspension and establish the content determination method of puerarin with the ultraviolet spectrophotometry. Methods： With Puerarin （main drug）,Avicel CL611 , poloxamer 188 and sodium dodecyl sulfate （SDS）, the prescription of Puerarin dry suspension was designed and optimized by orthogonal experiments. The content of puerarin was determined by ultraviolet spectrophotometry. Results ：The prescription is reasonable and reliable. The linearity range of Puerarin determined in the suspension was from 0.00303mg/ml to 0.0101mg/ml. The average recovery rate was 99. 6 0 % （RSD = 2.38%/^）. Conclusion： The preparation optimized was controllable with good repeatability. The content determination method was convenient and accurate with good specificity, which could be used in the quality control of puerarin dry suspension.