鲍曼不动杆菌耐药性与耐药基因分析

Analysis of Drug Resistance and Resistance Gene of Acinetobacter Baumannii

目的探讨临床分离鲍曼不动杆菌耐药性和耐药机制。方法对河南省某医院在2014年—2015年间分离的鲍曼不动杆菌临床株耐药性进行检测和分析;PCR检测gyr A,qnr B基因。结果 2014年—2015年间,氟喹诺酮类耐药鲍曼不动杆菌检出率最高为87.27%,碳靑霉烯类耐药的鲍曼不动杆菌耐药检出率为34.21%,以痰标本分离鲍曼不动杆菌为最多,占69.3%。氟喹诺酮类耐药鲍曼不动杆菌中,其gyr A基因检测2/5株有基因有突变,qnr B基因未检出。结论 2014年—2015年间,该院氟喹诺酮类耐药鲍曼不动杆菌检出率高(87.27%),耐药机制与gyr A基因突变有关。碳靑霉烯类耐药的鲍曼不动杆菌检出率为34.21%。

Objective To explore the drug resistance and mechanism of Acinetobacter baumannii from clinical isolates bacteria. Method The drug resistance of Acinetobacter baumannii isolated from a hospital in Henan Province from 2014 to 2015 were detected and analyzed, the gyrA gene and qnrB gene were detected by PCR. Results The detection rate of Acinetobacter baumannii to fluoroquinolone resistance was highest （87. 27% ） from 2014 to 2015. The detection rate of Acinetobacter baumannii to carbopenems drug resistance was 34. 21% . The number of Acinetobacter baumannii isolated from sputum specimens was the most（69. 3% ） in all specimens . There were two gyrA gene mutation in five gyrA gene of Acinetobacter baumannii to Fluoroquinolone resistant. There were no qnrB gene in five Acinetobacter baumannii to Fluoroquinolone resistant. Conclusion The detection rate of Acinetobacter baumannii to fluoroquinolone resistance is highest from 2014 to 2015. The one of the mechanisms of drug resistance is gyrA gene mutation. The detection rate of Acinetobacter baumannii to carbopenems drug resistance is 34. 21% .